Abstract：
Gene drive elements promote the spread of linked traits and can be used to change the composition or fate of wild populations. Cleave and Rescue (ClvR) drive elements sit at a fixed chromosomal position and include a DNA sequence-modifying enzyme such as Cas9/gRNAs that disrupts endogenous versions of an essential gene and a recoded version of the essential gene resistant to cleavage. ClvR spreads by creating conditions in which those lacking ClvR die because they lack functional versions of the essential gene. Here we demonstrate the essential features of the ClvR gene drive in the plant Arabidopsis thaliana through killing of gametes that fail to inherit a ClvR that targets the essential gene YKT61. Resistant alleles, which can slow or prevent drive, were not observed. Modelling shows plant ClvRs are robust to certain failure modes and can be used to rapidly drive population modification or suppression. Possible applications are discussed.
摘要：
基因驱动元件促进连锁性状的传播，可用于改变野生种群的组成或命运。切割和救援（ClvR）驱动元件位于固定的染色体位置，并包括DNA序列修饰酶，例如Cas9/gRNA，其破坏必需基因的内源版本和抗切割的必需基因的重新编码版本。ClvR通过创造条件来传播，在这些条件中，缺乏ClvR的人因为缺乏必需基因的功能版本而死亡。在这里，我们通过杀死未能继承针对必需基因YKT61的ClvR的配子来证明植物拟南芥中ClvR基因驱动的基本特征。抗性等位基因,可以减慢或阻止驱动器，没有被观察到。建模表明，工厂ClvR对某些故障模式具有鲁棒性，可用于快速驱动种群修改或抑制。讨论了可能的应用。
