PDF(Pubmed)
Abstract：
The current investigation aims to study the embryonic dermis formed in the early stages of development and identify the initial interstitial components of the dermis that serve as biological and structural scaffolds for the development of the dermal tissue. To investigate the dermal structure, the current study used morphological and immunological techniques. TCs identified by TEM. They had a cell body and unique podomeres and podoms. They formed a 3D network spread throughout the dermis. Homocellular contact established between them, as well as heterocellular contacts with other cells. Immunohistochemical techniques using specific markers for TCss CD34, CD117, and VEGF confirmed TC identification. TCs represent the major interstitial component in the dermal tissue. They established a 3D network, enclosing other cells and structures. Expression of VEGF by TC promotes angiogenesis. TCs establish cellular contact with sprouting endothelial cells. At the site of cell junction with TCs, cytoskeletal filaments identified and observed to form the pseudopodium core that projects from endothelial cells. TCs had proteolytic properties that expressed MMP-9, CD68, and CD21. Proteolytic activity aids in the removal of components of the extracellular matrix and the phagocytosis of degraded remnants to create spaces to facilitate the development of new dermal structures. In conclusion, TCs organized the scaffold for the development of future dermal structures, including fibrous components and skin appendages. Studying dermal TCs would be interested in the possibility of developing therapeutic strategies for treating different skin disorders and diseases.
摘要：
当前的调查旨在研究在发育早期形成的胚胎真皮，并确定真皮的初始间质成分，这些成分可作为真皮组织发育的生物和结构支架。为了研究真皮结构，目前的研究使用形态学和免疫学技术。通过TEM鉴定的TC。他们有一个细胞体和独特的食宿和食宿。它们形成了遍布真皮的3D网络。它们之间建立了同质细胞接触，以及与其他细胞的异型细胞接触。使用TCssCD34、CD117和VEGF的特异性标志物的免疫组织化学技术证实了TC鉴定。TC代表真皮组织中的主要间质成分。他们建立了一个3D网络，封闭其他细胞和结构。TC表达VEGF促进血管生成。TC与发芽内皮细胞建立细胞接触。在细胞与TC连接的位置，确定并观察到细胞骨架细丝形成从内皮细胞突出的假足核。TC具有表达MMP-9、CD68和CD21的蛋白水解特性。蛋白水解活性有助于去除细胞外基质的成分和吞噬降解的残余物以创造空间以促进新的真皮结构的发展。总之,TC组织了未来真皮结构发展的支架，包括纤维成分和皮肤附件。研究皮肤TC将对开发用于治疗不同皮肤病症和疾病的治疗策略的可能性感兴趣。
