Abstract：
The crude polysaccharide of Bletilla striata in this study was extracted by water extraction and alcohol precipitation and further purified by gel column to yield the purified component Bletilla striata polysaccharide (BSP). Its structure and innate immune regulation activity were studied. BSP mainly comprises mannose and glucose, with a monosaccharide molar ratio of 2.9:1 and a weight-average molecular weight of 28,365 Da. It is a new low-molecular-weight water-soluble neutral glucomannan. BSP contains a → 6)-β-Manp-(1→, →4)-β-Glcp-(1→, →4)-β-Manp-(1 → and →3)-α-Manp-(1 → linear main chain, containing β-Glcp-(1 → and β-Manp-(1 → two branched chain fragments were connected to the Man residue at position 4. BSP can enhance the anti-infection ability of Caenorhabditis elegans against Pseudomonas aeruginosa, significantly improve the phagocytic ability of RAW264.7 macrophages, stimulate the secretion of NO and TNF-α, and have good innate immune regulation activity. These findings guide the use of Bletilla striata polysaccharides with immunomodulatory action.
摘要：
本研究采用水提醇沉法提取白及粗多糖，并通过凝胶柱进一步纯化，得到纯化成分白及多糖（Bleetillastylatatopharm，BSP）。研究了其结构和先天免疫调节活性。BSP主要包括甘露糖和葡萄糖，单糖摩尔比为2.9:1,重均分子量为28,365Da。它是一种新型的低分子量水溶性中性葡甘露聚糖。BSP包含a→6)-β-Manp-(1→，→4)-β-Glcp-(1→，→4)-β-Manp-(1→和→3)-α-Manp-(1→线性主链，含有β-Glcp-（1→和β-Manp-（1→两个支链片段与位置4的Man残基连接。BSP可增强秀丽隐杆线虫对铜绿假单胞菌的抗感染能力,显著提高RAW264.7巨噬细胞的吞噬能力，刺激NO和TNF-α的分泌，并具有良好的先天免疫调节活性。这些发现指导了具有免疫调节作用的白及多糖的使用。
