PDF(Pubmed)
Abstract：
BACKGROUND: Fusarium and allied genera (fusarioid) species are common colonizers of roots and aerial plant parts, or act as phytopathogens in forestry and horticultural or grain crops. However, they can also cause a wide range of infections in humans, including onychomycosis, cutaneous and invasive infections. Fusarioid keratitis is characterized by an infection of the cornea with a suppurative and ulcerative appearance, which may cause damage to vision and permanent blindness. The aim of the present study was to investigate the prevalence of fusarioid species, biofilm formation and antifungal susceptibility profiling of clinical isolates recovered from patients with keratitis and dermatomycoses.
RESULTS: The study was performed between March, 2012-December, 2022. Demographic, clinical and epidemiological data of patients were also collected. In the present study, most of the patients with keratitis were male (74%), had a median age of 42 years old, worked with plant material or debris and 26% of them reported eye trauma. Regarding dermatomycosis, most of patients were female and exhibited toenail lesions. Forty-seven isolates belonged to the genus Neocosmospora (78.33%), nine to the Fusarium fujikuroi (15%) and four to the Fusarium oxysporum (6.66%) species complexes. Several strains were moderate biofilm producers, specifically among Fusarium annulatum. Most strains showed increased MICs to amphotericin B and ketoconazole and low MICs to itraconazole. MICs ranged from 0.25 to 16 μg/mL for amphotericin B, 0.0625 to >16 μg/mL for ketoconazole and 0.125 to 8 for itraconazole.
CONCLUSIONS: It is possible to conclude that fusarioid keratitis in Northeastern Brazil is an important and neglected disease, given the high number of cases, increased need for keratoplasty and poor outcome of the disease.
RESULTS: The study was performed between March, 2012-December, 2022. Demographic, clinical and epidemiological data of patients were also collected. In the present study, most of the patients with keratitis were male (74%), had a median age of 42 years old, worked with plant material or debris and 26% of them reported eye trauma. Regarding dermatomycosis, most of patients were female and exhibited toenail lesions. Forty-seven isolates belonged to the genus Neocosmospora (78.33%), nine to the Fusarium fujikuroi (15%) and four to the Fusarium oxysporum (6.66%) species complexes. Several strains were moderate biofilm producers, specifically among Fusarium annulatum. Most strains showed increased MICs to amphotericin B and ketoconazole and low MICs to itraconazole. MICs ranged from 0.25 to 16 μg/mL for amphotericin B, 0.0625 to >16 μg/mL for ketoconazole and 0.125 to 8 for itraconazole.
CONCLUSIONS: It is possible to conclude that fusarioid keratitis in Northeastern Brazil is an important and neglected disease, given the high number of cases, increased need for keratoplasty and poor outcome of the disease.
摘要：
背景：镰刀菌和相关属（镰刀菌）物种是根和地上植物部分的常见定殖者，或在林业和园艺或粮食作物中充当植物病原体。然而,它们还可以在人类中引起广泛的感染，包括甲癣,皮肤和侵入性感染。镰刀状角膜炎的特征是角膜感染，具有化脓性和溃疡性外观，这可能会导致视力受损和永久性失明。本研究的目的是调查镰刀菌的流行情况，从角膜炎和皮肤真菌病患者中恢复的临床分离株的生物膜形成和抗真菌药敏分析。
结果：该研究在3月之间进行，2012-12月,2022年。人口统计,同时收集患者的临床和流行病学资料.在本研究中,大多数角膜炎患者是男性（74%），平均年龄为42岁，与植物材料或碎片一起工作，其中26％报告了眼外伤。关于皮肤真菌病，大多数患者为女性，并表现出趾甲病变。47个分离株属于新孢菌属(78.33%),9种是富士镰刀菌（15％），4种是尖孢镰刀菌（6.66％）。几个菌株是中度生物膜生产者，特别是在环状镰刀菌中。大多数菌株对两性霉素B和酮康唑的MIC增加，对伊曲康唑的MIC低。两性霉素B的MIC范围为0.25至16μg/mL，酮康唑为0.0625至>16μg/mL,伊曲康唑为0.125至8。
结论：可以得出结论，巴西东北部的镰刀状角膜炎是一种重要且被忽视的疾病，鉴于案件数量众多，角膜移植术的需求增加和疾病的不良预后。
结果：该研究在3月之间进行，2012-12月,2022年。人口统计,同时收集患者的临床和流行病学资料.在本研究中,大多数角膜炎患者是男性（74%），平均年龄为42岁，与植物材料或碎片一起工作，其中26％报告了眼外伤。关于皮肤真菌病，大多数患者为女性，并表现出趾甲病变。47个分离株属于新孢菌属(78.33%),9种是富士镰刀菌（15％），4种是尖孢镰刀菌（6.66％）。几个菌株是中度生物膜生产者，特别是在环状镰刀菌中。大多数菌株对两性霉素B和酮康唑的MIC增加，对伊曲康唑的MIC低。两性霉素B的MIC范围为0.25至16μg/mL，酮康唑为0.0625至>16μg/mL,伊曲康唑为0.125至8。
结论：可以得出结论，巴西东北部的镰刀状角膜炎是一种重要且被忽视的疾病，鉴于案件数量众多，角膜移植术的需求增加和疾病的不良预后。
