关键词: Macrolides Multiplex PCRMultiplex PCR Polymerase chain reaction Resistance determinants Tetracyclines

Mesh : Macrolides / pharmacology Polymerase Chain Reaction / methods Humans Anti-Bacterial Agents / pharmacology Tetracycline / pharmacology Drug Resistance, Bacterial / genetics Animals DNA, Bacterial / genetics Microbial Sensitivity Tests / methods

来  源:   DOI:10.1007/978-1-0716-3898-9_6

Abstract:
Globally, the main molecular trials being developed to study the genetic determinants responsible for conferring resistance to bacterial organisms are amplification-based methods, hybridization-based methods, and sequence-based methods. In the specific case of Streptococcus suis, polymerase chain reaction is the only test tuned up until now for detecting resistant clinical isolates to macrolides and/or tetracyclines, the two main groups of antibiotics being ineffective against this human and animal pathogen.
摘要:
全球范围内,正在开发的研究负责赋予细菌生物抗性的遗传决定因素的主要分子试验是基于扩增的方法,基于杂交的方法,和基于序列的方法。在猪链球菌的具体情况下,聚合酶链反应是迄今为止唯一一种用于检测对大环内酯类和/或四环素类耐药的临床分离株的检测方法,两种主要的抗生素对这种人类和动物病原体无效。
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