PDF(Pubmed)
Abstract：
Dengue fever poses a significant global health threat, with symptoms including dengue hemorrhagic fever and dengue shock syndrome. Each year, India experiences fatal dengue outbreaks with severe manifestations. The primary cause of severe inflammatory responses in dengue is a cytokine storm. Individuals with a secondary dengue infection of a different serotype face an increased risk of complications due to antibody-dependent enhancement. Therefore, it is crucial to identify potential risk factors and biomarkers for effective disease management. In the current study, we assessed the prevalence of dengue infection in and around Aligarh, India, and explored the role of cytokines, including CXCL5, CXCL9, and CCL17, in primary and secondary dengue infections, correlating them with various clinical indices. Among 1,500 suspected cases, 367 tested positive for dengue using Real-Time PCR and ELISA. In secondary dengue infections, the serum levels of CXCL5, CXCL9, and CCL17 were significantly higher than in primary infections (P < 0.05). Dengue virus (DENV)-2 showed the highest concentrations of CXCL5 and CCL17, whereas DENV-1 showed the highest concentrations of CXCL9. Early detection of these cytokines could serve as potential biomarkers for diagnosing severe dengue, and downregulation of these cytokines may prove beneficial for the treatment of severe dengue infections.
摘要：
登革热对全球健康构成重大威胁，症状包括登革热出血热和登革热休克综合征。每一年,印度经历了严重表现的致命登革热暴发。登革热中严重炎症反应的主要原因是细胞因子风暴。具有不同血清型的继发性登革热感染的个体由于抗体依赖性增强而面临并发症的风险增加。因此,确定潜在的危险因素和生物标志物对于有效的疾病管理至关重要.在目前的研究中,我们评估了Aligarh及其周围地区的登革热感染率，印度,探索细胞因子的作用，包括CXCL5、CXCL9和CCL17，在原发性和继发性登革热感染中，将它们与各种临床指标相关联。在1500例疑似病例中，367使用实时PCR和ELISA检测登革热呈阳性。在继发性登革热感染中，血清CXCL5、CXCL9和CCL17水平明显高于原发感染组(P<0.05)。登革病毒(DENV)-2显示最高浓度的CXCL5和CCL17,而DENV-1显示最高浓度的CXCL9。早期检测这些细胞因子可以作为诊断严重登革热的潜在生物标志物。这些细胞因子的下调可能对严重登革热感染的治疗有益。
