PDF(Pubmed)
Abstract：
UNASSIGNED: Estrogen homeostasis is crucial for bladder function, and estrogen deprivation resulting from menopause, ovariectomy or ovarian dysfunction may lead to various bladder dysfunctions. However, the specific mechanisms are not fully understood.
UNASSIGNED: We simulated estrogen deprivation using a rat ovariectomy model and supplemented estrogen through subcutaneous injections. The metabolic characteristics of bladder tissue were analyzed using non-targeted metabolomics, followed by bioinformatics analysis to preliminarily reveal the association between estrogen deprivation and bladder function.
UNASSIGNED: We successfully established a rat model with estrogen deprivation and, through multivariate analysis and validation, identified several promising biomarkers represented by 3, 5-tetradecadiencarnitine, lysoPC (15:0), and cortisol. Furthermore, we explored estrogen deprivation-related metabolic changes in the bladder primarily characterized by amino acid metabolism imbalance.
UNASSIGNED: This study, for the first time, depicts the metabolic landscape of bladder resulting from estrogen deprivation, providing an important experimental basis for future research on bladder dysfunctions caused by menopause.
UNASSIGNED: We simulated estrogen deprivation using a rat ovariectomy model and supplemented estrogen through subcutaneous injections. The metabolic characteristics of bladder tissue were analyzed using non-targeted metabolomics, followed by bioinformatics analysis to preliminarily reveal the association between estrogen deprivation and bladder function.
UNASSIGNED: We successfully established a rat model with estrogen deprivation and, through multivariate analysis and validation, identified several promising biomarkers represented by 3, 5-tetradecadiencarnitine, lysoPC (15:0), and cortisol. Furthermore, we explored estrogen deprivation-related metabolic changes in the bladder primarily characterized by amino acid metabolism imbalance.
UNASSIGNED: This study, for the first time, depicts the metabolic landscape of bladder resulting from estrogen deprivation, providing an important experimental basis for future research on bladder dysfunctions caused by menopause.
摘要：
■雌激素稳态对膀胱功能至关重要，更年期导致的雌激素剥夺，卵巢切除术或卵巢功能障碍可能导致各种膀胱功能障碍。然而,具体机制尚未完全理解。
■我们使用大鼠卵巢切除模型并通过皮下注射补充雌激素来模拟雌激素剥夺。采用非靶向代谢组学方法分析膀胱组织的代谢特征,随后通过生物信息学分析，初步揭示雌激素剥夺与膀胱功能之间的关系。
■我们成功建立了雌激素剥夺大鼠模型,通过多变量分析和验证，确定了几种有希望的生物标志物代表3，5-十四碳二烯卡尼汀，lysoPC(15:0)，和皮质醇。此外，我们探讨了以氨基酸代谢失衡为主要特征的膀胱雌激素剥夺相关代谢变化.
■这项研究，第一次,描绘了由于雌激素剥夺而导致的膀胱代谢景观，为今后更年期引起的膀胱功能障碍的研究提供了重要的实验依据。
■我们使用大鼠卵巢切除模型并通过皮下注射补充雌激素来模拟雌激素剥夺。采用非靶向代谢组学方法分析膀胱组织的代谢特征,随后通过生物信息学分析，初步揭示雌激素剥夺与膀胱功能之间的关系。
■我们成功建立了雌激素剥夺大鼠模型,通过多变量分析和验证，确定了几种有希望的生物标志物代表3，5-十四碳二烯卡尼汀，lysoPC(15:0)，和皮质醇。此外，我们探讨了以氨基酸代谢失衡为主要特征的膀胱雌激素剥夺相关代谢变化.
■这项研究，第一次,描绘了由于雌激素剥夺而导致的膀胱代谢景观，为今后更年期引起的膀胱功能障碍的研究提供了重要的实验依据。
