PDF(Pubmed)
Abstract：
Follicle culture is a process of dividing follicle unit structures from ovaries for continued culture in vitro in an incubator, which simulates the in vivo environment. Alginate gel is the most stable and most convenient 3D material currently used in follicle culture. We performed in vitro follicle culture following the standard operating procedure recommended by the Follicle Handbook and we have summarized our experience and skills in details. Through several experiments, we found only follicles tightly surrounded by theca cells can grow healthily until the preovulatory stage. In addition, the hardness of alginate gel is crucial for constructing the 3D culture system, and selecting appropriate tools can reduce damage to the alginate gel and shorten the time follicles are exposed to room temperature. Our detailed operation improves bioavailability and provides a more natural environment for the entire process of follicular growth.•Alginate gel is still the most suitable 3D material used for in vitro follicle culture.•Follicle integrity and the hardness of alginate gel are the keys for in vitro culture.•Detailed operation steps better protect the follicular microenvironment and improve bioavailability.
摘要：
卵泡培养是指从卵巢中分割卵泡单位结构，在培养箱中进行体外持续培养，模拟体内环境。海藻酸盐凝胶是目前用于卵泡培养的最稳定和最方便的3D材料。我们按照《毛囊手册》推荐的标准操作程序进行了体外卵泡培养，并详细总结了我们的经验和技能。通过几个实验,我们发现，只有被卵泡膜细胞紧密包围的卵泡才能健康生长，直到排卵前阶段。此外,藻酸盐凝胶的硬度对于构建3D培养系统至关重要，选择合适的工具可以减少藻酸盐凝胶的损伤，缩短卵泡暴露在室温下的时间。我们的详细操作提高了生物利用度，并为卵泡生长的整个过程提供了更自然的环境。•藻酸盐凝胶仍然是用于体外卵泡培养的最合适的3D材料。•毛囊完整性和藻酸盐凝胶的硬度是体外培养的关键。•详细的操作步骤更好地保护卵泡微环境并提高生物利用度。
