PDF(Pubmed)
Abstract：
Cancer is a disease resulting from the disruption of cell cycle regulation, leading to the abnormal and unchecked proliferation of cells. Medicinal plants are rich in various bioactive phytochemicals or nutritional compounds. The aim is to determine the cytotoxic and genotoxic effects of ethanolic extracts of Macaranga peltata leaves on human oral cancer cell lines. The study setting was centre for Research on Molecular and Applied Sciences, Azeezia College of Dental Sciences and Research. The study design is a Comparative In Vitro study. Shade dried leaves of Macaranga peltata were subjected to Soxhlet extraction, and ethanolic extract was prepared. In vitro cytotoxic effects on human oral cancer cell lines were evaluated by (3-(4,5-dimethyl thiazole-2yl)-2,5-diphenyl tetrazolium bromide) MTT assay, and genotoxic effect was evaluated by comet assay. Untreated cell lines were used as control, and 5-fluorouracil was used as positive control. All experiments were performed in triplicates, and results were represented as Mean+/- SE. One-way ANOVA and Dunnet test were performed to analyze data. ***P < 0.001 compared with the control group. The ethanolic extract of Macaranga peltata exhibited cytotoxicity against oral cancer cells (LC50: 40.193089 μg/ml). There was a concentration-dependent increase in cell death, and at 100 μg/ml, the extract was most effective, causing 50% inhibition of viability. The comet assay showed significant genotoxic effects compared with 5-fluorouracil and untreated oral cancer cell lines. The ethanolic extract of Macaranga peltata leaves was subjected to MTT assay and comet using KB cell lines. The study concludes that the extract gave promising result for the anticancer activity on the KB cell lines producing cytotoxicity and genotoxicity.
摘要：
癌症是一种由细胞周期调节中断引起的疾病，导致细胞的异常和无控制的增殖。药用植物富含各种生物活性植物化学物质或营养化合物。目的是确定Macarangapeltata叶的乙醇提取物对人口腔癌细胞系的细胞毒性和遗传毒性作用。研究环境是分子和应用科学研究中心，Azeezia牙科科学与研究学院。研究设计是体外比较研究。将Macarangapeltata的阴影干叶进行索氏提取，制备乙醇提取物。通过（3-（4,5-二甲基噻唑-2基）-2,5-二苯基四唑溴化物）MTT测定法评估对人口腔癌细胞系的体外细胞毒性作用，并通过彗星试验评估遗传毒性作用。未处理的细胞系用作对照,5-氟尿嘧啶用作阳性对照。所有实验一式三份进行，结果表示为平均值+/-SE。进行单因素方差分析和Dunnet检验来分析数据。与对照组相比，***P<0.001。Macarangapeltata的乙醇提取物对口腔癌细胞表现出细胞毒性（LC50：40.193089μg/ml）。细胞死亡的浓度依赖性增加，在100μg/ml时，提取物最有效，导致50%的活力抑制。与5-氟尿嘧啶和未经处理的口腔癌细胞系相比，彗星测定法显示出显着的遗传毒性作用。对Macarangapeltata叶的乙醇提取物进行MTT测定并使用KB细胞系进行彗星。该研究得出结论，该提取物对产生细胞毒性和遗传毒性的KB细胞系的抗癌活性产生了有希望的结果。
