PDF(Pubmed)
Abstract：
UNASSIGNED: This study aimed to investigate the antibacterial effects of plasma-activated saline (PAS) on My-cobacterium tuberculosis (Mtb).
UNASSIGNED: We conducted a growth assay on 3 strains of Mtb and an antibiotic sensitivity test on 4 strains of Mtb. Both tests included groups treated with normal saline (NS), PAS, and hydrochloric acid (HCl). The test of antibiotic sensitivity consisted of parallel tests with two concentrations of bacteria suspension: 10-2 and 10-4. The selected antibiotics were rifampicin (RIF), isoniazid (INH), ethambutol (EMB), and streptomycin (SM). The number of bacteria was determined after one month of culture under different conditions. The Kruskal-Wallis test was used to analyze the differences in grouping factors at representative time points.
UNASSIGNED: The growth assay indicated that PAS significantly inhibited the growth of 3 strains of Mtb compared with NS and HCl treatment groups. Furthermore, except for the initial observation time point, the remaining three observation time points consistently demonstrate no significant differences between the NS group and the HCl group. The antibiotic sensitivity test of INH, SM, and RIF indicated that PAS could inhibit the growth of antibiotic-resistant Mtb, and the antibiotic sensitivity test of INH and SM with bacterial suspension concentration of 10-2 and SM with bacterial suspension concentration of 10-4 showed statistically different results. The antibiotic sensitivity test of EMB indicated that the growth of Mtb in PAS was slower than that in NS and HCl in both antibiotic-resistant and sensitive Mtb, but there was no statistical difference.
UNASSIGNED: The study indicates that PAS contains a significant amount of active substances and exhibits high oxidizability and an acidic pH state. The unique physicochemical properties of PAS significantly delayed the growth of Mtb, compared to the NS and the HCl. PAS not only inhibited the growth of drug-sensitive strains but also significantly enhanced the sensitivity of drug-resistant strains to anti-tuberculosis drugs, which may provide a new therapeutic strategy for the treatment of tuberculosis.
UNASSIGNED: We conducted a growth assay on 3 strains of Mtb and an antibiotic sensitivity test on 4 strains of Mtb. Both tests included groups treated with normal saline (NS), PAS, and hydrochloric acid (HCl). The test of antibiotic sensitivity consisted of parallel tests with two concentrations of bacteria suspension: 10-2 and 10-4. The selected antibiotics were rifampicin (RIF), isoniazid (INH), ethambutol (EMB), and streptomycin (SM). The number of bacteria was determined after one month of culture under different conditions. The Kruskal-Wallis test was used to analyze the differences in grouping factors at representative time points.
UNASSIGNED: The growth assay indicated that PAS significantly inhibited the growth of 3 strains of Mtb compared with NS and HCl treatment groups. Furthermore, except for the initial observation time point, the remaining three observation time points consistently demonstrate no significant differences between the NS group and the HCl group. The antibiotic sensitivity test of INH, SM, and RIF indicated that PAS could inhibit the growth of antibiotic-resistant Mtb, and the antibiotic sensitivity test of INH and SM with bacterial suspension concentration of 10-2 and SM with bacterial suspension concentration of 10-4 showed statistically different results. The antibiotic sensitivity test of EMB indicated that the growth of Mtb in PAS was slower than that in NS and HCl in both antibiotic-resistant and sensitive Mtb, but there was no statistical difference.
UNASSIGNED: The study indicates that PAS contains a significant amount of active substances and exhibits high oxidizability and an acidic pH state. The unique physicochemical properties of PAS significantly delayed the growth of Mtb, compared to the NS and the HCl. PAS not only inhibited the growth of drug-sensitive strains but also significantly enhanced the sensitivity of drug-resistant strains to anti-tuberculosis drugs, which may provide a new therapeutic strategy for the treatment of tuberculosis.
摘要：
■本研究旨在研究血浆活化盐水（PAS）对结核分枝杆菌（Mtb）的抗菌作用。
■我们对3株Mtb进行了生长测定，对4株Mtb进行了抗生素敏感性测试。两项测试包括用生理盐水（NS）治疗的组，PAS,和盐酸(HCl)。抗生素敏感性测试包括两种浓度的细菌悬浮液的平行测试:10-2和10-4。选择的抗生素是利福平（RIF），异烟肼（异烟肼），乙胺丁醇(EMB),和链霉素(SM)。在不同条件下培养一个月后测定细菌数量。使用Kruskal-Wallis检验分析代表性时间点分组因素的差异。
■生长测定表明，与NS和HCl处理组相比，PAS显着抑制了3株Mtb的生长。此外，除了最初的观测时间点，其余三个观察时间点一致显示NS组和HCl组之间无显著差异.INH的抗生素敏感性试验，SM,和RIF表明PAS可以抑制抗生素抗性Mtb的生长，细菌悬浮液浓度为10-2的INH和SM以及细菌悬浮液浓度为10-4的SM的抗生素敏感性试验显示出统计学差异。EMB的抗生素敏感性试验表明，在耐药和敏感的Mtb中，PAS中Mtb的生长均慢于NS和HCl中。但没有统计学差异。
■研究表明，PAS含有大量的活性物质，并表现出高的氧化性和酸性pH状态。PAS独特的理化性质显著延缓了Mtb的生长,与NS和HCl相比。PAS不仅抑制了药物敏感菌株的生长,而且显著增强了耐药菌株对抗结核药物的敏感性,这可能为结核病的治疗提供新的治疗策略。
■我们对3株Mtb进行了生长测定，对4株Mtb进行了抗生素敏感性测试。两项测试包括用生理盐水（NS）治疗的组，PAS,和盐酸(HCl)。抗生素敏感性测试包括两种浓度的细菌悬浮液的平行测试:10-2和10-4。选择的抗生素是利福平（RIF），异烟肼（异烟肼），乙胺丁醇(EMB),和链霉素(SM)。在不同条件下培养一个月后测定细菌数量。使用Kruskal-Wallis检验分析代表性时间点分组因素的差异。
■生长测定表明，与NS和HCl处理组相比，PAS显着抑制了3株Mtb的生长。此外，除了最初的观测时间点，其余三个观察时间点一致显示NS组和HCl组之间无显著差异.INH的抗生素敏感性试验，SM,和RIF表明PAS可以抑制抗生素抗性Mtb的生长，细菌悬浮液浓度为10-2的INH和SM以及细菌悬浮液浓度为10-4的SM的抗生素敏感性试验显示出统计学差异。EMB的抗生素敏感性试验表明，在耐药和敏感的Mtb中，PAS中Mtb的生长均慢于NS和HCl中。但没有统计学差异。
■研究表明，PAS含有大量的活性物质，并表现出高的氧化性和酸性pH状态。PAS独特的理化性质显著延缓了Mtb的生长,与NS和HCl相比。PAS不仅抑制了药物敏感菌株的生长,而且显著增强了耐药菌株对抗结核药物的敏感性,这可能为结核病的治疗提供新的治疗策略。
