PDF(Pubmed)
Abstract：
Postbiotics have various functional effects, such as antioxidant, anti-inflammatory, and anti-obesity. Levilactobacillus brevis BK3, the subject of this study, was derived from lactic acid bacteria isolated from Kimchi, a traditional Korean fermented food. The antioxidant activity of BK3 was confirmed through the measurements of 2,2-diphenyl-1-picryl-hydrazyl (DPPH), 2,2\'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and total antioxidant capacity (TAC). The wrinkle improvement effect was validated by assessing elastase inhibitory activity and collagenase inhibitory activity. The intracellular activity was confirmed using human keratinocytes (HaCaT) and human fibroblasts (HFF-1). BK3 protects skin cells from oxidative stress induced by H2O2 and reduces intracellular reactive oxygen species (ROS) production. In addition, the expressions of the antioxidant genes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were upregulated. Meanwhile, matrix metalloproteinase-1 (MMP-1) and collagen type I alpha 1 (COL1A1), involved in collagen degradation and synthesis, were significantly regulated. These results suggest the possibility of utilizing BK3 as a functional ingredient with antioxidant and wrinkle-improving effects.
摘要：
益生菌具有多种功能作用，如抗氧化剂，抗炎,和抗肥胖。短小左半芽孢杆菌BK3，本研究的主题，来自从泡菜中分离的乳酸菌，一种传统的韩国发酵食品。BK3的抗氧化活性通过2,2-二苯基-1-吡啶-肼基(DPPH)的测量得到证实,2,2'-氮杂-双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)，和总抗氧化能力(TAC)。通过评估弹性蛋白酶抑制活性和胶原酶抑制活性来验证皱纹改善效果。使用人角质形成细胞(HaCaT)和人成纤维细胞(HFF-1)确认细胞内活性。BK3保护皮肤细胞免受H2O2诱导的氧化应激并减少细胞内ROS的产生。此外,抗氧化基因超氧化物歧化酶（SOD）的表达，过氧化氢酶(CAT),谷胱甘肽过氧化物酶（GPx）上调。同时,基质金属蛋白酶-1（MMP-1）和I型胶原α1（COL1A1），参与胶原蛋白的降解和合成，受到显著监管。这些结果表明利用BK3作为具有抗氧化和改善皱纹效果的功能成分的可能性。
