Abstract：
Toxoplasmosis is a foodborne disease caused by the protozoan Toxoplasma gondii, and transmitted to humans by eating raw or undercooked meat, mainly. Poultry, beef, and pork are the main meats consumed in Peru; despite this, guinea pig meat is also widely consumed. For this reason, the objective of this study was to molecularly detect T. gondii in domestic and wild guinea pigs from the Marangani district in Cuzco, Peru, and identify some risk factors associated with this pathogen. DNA was extracted from the brain tissue samples of guinea pigs (30 domestic and 30 wild), and PCR protocols were used to amplify the internal transcribed spacer (ITS-1) region and a 529 bp fragment from the T. gondii genome. T. gondii DNA was detected in 14 (23.3%) guinea pigs. T. gondii frequency was 33.3% in domestic guinea pigs and 13.3% in wild guinea pigs. Our results demonstrated that guinea pigs represent an important source for T. gondii infection in human populations in this locality.
摘要：
弓形虫病是由弓形虫原虫引起的食源性疾病，并通过食用生肉或未煮熟的肉传播给人类，主要是。家禽,牛肉,猪肉是秘鲁消费的主要肉类；尽管如此，豚鼠肉也被广泛食用。出于这个原因,这项研究的目的是对库斯科马兰加尼地区的家养和野生豚鼠中的弓形虫进行分子检测，秘鲁,并确定与该病原体相关的一些危险因素。从豚鼠(30只国产和30只野生)的脑组织样本中提取DNA，和PCR方案用于从弓形虫基因组中扩增内部转录间隔区（ITS-1）和529bp片段。在14只(23.3%)豚鼠中检测到弓形虫DNA。家养豚鼠的弓形虫频率为33.3%，野生豚鼠为13.3%。我们的结果表明，豚鼠是该地区人群中弓形虫感染的重要来源。
