Abstract：
Transcription factors play an important role in regulating the expression of detoxification genes (e.g. P450s) that confer insecticide resistance. Our previous study identified a series of candidate transcription factors (CYP6B7-fenvalerate association proteins, CAPs) that may be related to fenvalerate-induced expression of CYP6B7 in a field HDTJ strain of H. armigera. Whether these CAPs can mediate the transcript of CYP6B7 induced by fenvalerate in a susceptible HDS strain of H. armigera remains unknown. Further study showed that the expression levels of multiple CAPs were significantly induced by fenvalerate in HDS strain. Knockdown of CAP19 [fatty acid synthase-like (FAS)], CAP22 [polysaccharide biosynthesis domain-containing protein 1 (PBDC1)], CAP24 [5-formyltetrahydrofolate cycloligase (5-FCL)], CAP30 [peptidoglycan recognition protein LB-like (PGRP)] and CAP33 [NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (NDUFA11)] resulted in significant inhibition of CYP6B7 and some other P450 genes expression; meanwhile, the sensitivity of HDS strain larvae to fenvalerate was significantly increased. In addition, PBDC1, PGRP and NDUFA11, either alone or in combination, could significantly enhance the activity of CYP6B7 promoter in HDS strain, as well as the expression level of CYP6B7 gene in Sf9 cells line. These results suggested that PBDC1, PGRP and NDUFA11 may be involved in the transcript regulation of key detoxifying genes in response to fenvalerate in HDS strain of H. armigera.
摘要：
转录因子在调节赋予杀虫剂抗性的解毒基因(例如P450)的表达中起重要作用。我们先前的研究确定了一系列候选转录因子（CYP6B7-氰戊菊酯缔合蛋白，CAPs）可能与氰戊菊酯诱导的CYP6B7在棉铃虫的田间HDTJ品系中的表达有关。这些CAPs是否可以介导氰戊菊酯诱导的CYP6B7在棉铃虫易感HDS品系中的转录物仍然未知。进一步研究表明，氰戊菊酯在HDS菌株中显著诱导了多种CAPs的表达水平。CAP19[脂肪酸合成酶样（FAS）]的敲低，CAP22[含多糖生物合成结构域的蛋白1（PBDC1）]，CAP24[5-甲酰四氢叶酸环化酶（5-FCL）]，CAP30[肽聚糖识别蛋白LB样（PGRP）]和CAP33[NADH脱氢酶[泛醌]1α亚复合物亚基11（NDUFA11）]导致CYP6B7和其他一些P450基因表达的显着抑制；同时，HDS菌株幼虫对氰戊菊酯的敏感性显着提高。此外,PBDC1，PGRP和NDUFA11，单独或组合，能显著增强HDS菌株CYP6B7启动子的活性,以及CYP6B7基因在Sf9细胞系中的表达水平。这些结果表明，PBDC1，PGRP和NDUFA11可能参与了棉铃虫HDS株对氰戊菊酯的关键解毒基因的转录调控。
