Abstract：
The evolution of resistance to insecticides poses a significant threat to pest management programs. Understanding the molecular mechanisms underlying insecticide resistance is essential to design sustainable pest control and resistance management programs. The fall armyworm, Spodoptera frugiperda, is an important insect pest of many crops and has a remarkable ability to evolve resistance to insecticides. In this study, we employed bulk segregant analysis (BSA) combined with DNA and RNA sequencing to characterize the molecular basis of spinetoram resistance in S. frugiperda. Analysis of genomic data derived from spinetoram selected and unselected bulks and the spinetoram-resistant and susceptible parental strains led to the identification of a three-nucleotide deletion in the gene encoding the nicotinic acetylcholine receptor α6 subunit (nAChR α6). Transcriptome profiling identified the upregulation of few genes encoding detoxification enzymes associated with spinetoram resistance. Thus, spinetoram resistance in S. frugiperda appears to be mediated mainly by target site insensitivity with a minor role of detoxification enzymes. Our findings provide insight into the mechanisms underpinning resistance to spinetoram in S. frugiperda and will inform the development of strategies to control this highly damaging, globally distributed crop pest.
摘要：
对杀虫剂的抗性的演变对害虫管理计划构成了重大威胁。了解杀虫剂抗性的分子机制对于设计可持续的害虫控制和抗性管理计划至关重要。秋天的粘虫，节食夜蛾,是许多作物的重要害虫，具有明显的抗杀虫剂能力。在这项研究中,我们采用了批量分离分析（BSA）结合DNA和RNA测序来表征S.frugiperda中多辛抗性的分子基础。对来自选择的和未选择的多甲胆碱的基因组数据以及多甲胆碱抗性和易感的亲本菌株的基因组数据的分析导致了编码烟碱乙酰胆碱受体α6亚基（nAChRα6）的基因中三个核苷酸的缺失。转录组谱分析确定了少数编码解毒酶的基因的上调，这些解毒酶与多角菌抗性有关。因此，frugiperda中的多杀菌素抗性似乎主要是由靶位点不敏感性介导的，而解毒酶的作用较小。我们的发现提供了对S.frugiperda中对spinetoram的抗性的机制的见解，并将为控制这种高度破坏性的策略的制定提供信息，全球分布的农作物害虫。
