Abstract：
The efficacy of three antimicrobials was evaluated against two severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) surrogates - bovine coronavirus (BCoV) and human coronavirus (HCoV) OC43 - on hard and soft nonporous materials. Three antimicrobials with three different active ingredients (chlorine, hydrogen peroxide, and quaternary ammonium compound + alcohol) were studied. Initially, a neutralization method was optimized for each antimicrobial. Then, we determined their efficacy against BCoV and HCoV OC43 in both suspension and on surfaces made with polyethylene terephthalate (PET) plastic and vinyl upholstery fabric. All tests were conducted under ambient environmental conditions with a soil load of 5% fetal bovine serum. After a 2-min exposure, all three antimicrobials achieved a >3.0 log10 reduction in viral titers in suspension. All three also reduced virus infectivity on both surface materials below the detection limit (0.6 log10 TCID50/carrier). Treatments in which the reduction in virus titer was <3.0 log10 were attributed to a decreased dynamic range on the carrier during drying prior to disinfection. The carrier data revealed that both surrogates were inactivated more rapidly (p <0.05) on vinyl or under conditions of high relative humidity. Three classes of antimicrobials were efficacious against both SARS-CoV-2 surrogate viruses, with BCoV demonstrating slightly less sensitivity compared to HCoV OC43. These findings also illustrate the importance of (1) optimizing the neutralization method and (2) considering relative humidity as a key factor for efficacy testing.
摘要：
在硬质和软质无孔材料上评估了三种抗菌剂对两种严重急性呼吸道综合症冠状病毒2（SARS-CoV-2）替代品-牛冠状病毒（BCoV）和人冠状病毒（HCoV）OC43的疗效。具有三种不同活性成分（氯，过氧化氢,和季铵化合物+醇)进行了研究。最初，每种抗菌剂的中和方法进行了优化。然后，我们确定了它们在悬浮液中以及在用聚对苯二甲酸乙二醇酯（PET）塑料和乙烯基装饰织物制成的表面上对BCoV和HCoVOC43的功效。所有测试均在周围环境条件下进行，土壤负荷为5％胎牛血清。暴露2分钟后,所有三种抗微生物剂在悬浮液中的病毒滴度降低>3.0log10.所有这三者还将两种表面材料上的病毒感染性降低到检测极限(0.6log10TCID50/载体)以下。其中病毒滴度降低<3.0log10的处理归因于在消毒前干燥期间载体上的动态范围降低。载体数据显示,两种替代物在乙烯基上或在高相对湿度的条件下更快速地失活(p<0.05)。三类抗菌剂对两种SARS-CoV-2替代病毒均有效，与HCoVOC43相比，BCoV的灵敏度略低。这些发现还说明了(1)优化中和法和(2)考虑相对湿度作为功效测试的关键因素的重要性。
