Abstract：
Selenoproteins found in selenium (Se)-enriched vegetables play a vital role in maintaining human health. In this study, four Se-containing broccoli proteins (Se-BP: albumin, globulin, prolamin, and glutelin) were continuous extracted by Osborne method. Three ultrafiltered fractions were subsequently obtained from the glutelin hydrolysate, composed of Se-contained broccoli peptides (Se-Bp) with different molecular weights (MW), namely, < 1 kDa, 1-3 kDa, and 3-10 kDa. Glutelin exhibited the highest protein yield (65.60 ± 1.07%), purity (78.39 ± 0.95%), nutritional value, organic Se content (88.05 ± 0.32% of total Se content), and Se speciation distribution (selenocystine, selenomethionine, methylselenocysteine, and selenoethionine). Additionally, the antioxidant activity of different MW of Se-Bp was assessed using electron spin resonance spectroscopy. The results revealed that antioxidant activity of the candidate peptide is dependent upon its Se content, amino acid composition, and MW, especially Se-Bp with MW of 1-3 kDa displayed the strongest free radical scavenging ability.
摘要：
富含硒（Se）的蔬菜中的硒蛋白在维持人体健康中起着至关重要的作用。在这项研究中,四种含硒的西兰花蛋白（Se-BP：白蛋白，球蛋白,醇溶蛋白,和谷蛋白）通过奥斯本方法连续提取。随后从谷蛋白水解物中获得三个超滤级分，由不同分子量（MW）的含硒西兰花肽（Se-Bp）组成，即,<1kDa,1-3kDa,和3-10kDa。蛋白产量最高(65.60±1.07%),纯度（78.39±0.95%），营养价值,有机硒含量(总硒含量的88.05±0.32%)，和硒形态分布(硒半胱氨酸，硒代蛋氨酸，甲基硒代半胱氨酸，和硒代硫氨酸）。此外,使用电子自旋共振光谱法评估了不同MW的Se-Bp的抗氧化活性。结果表明，候选肽的抗氧化活性取决于其Se含量，氨基酸组成,和MW,特别是分子量为1-3kDa的Se-Bp表现出最强的自由基清除能力。
