PDF(Pubmed)
Abstract：
In this study, the complex organization of the AnG in the giant freshwater prawn Macrobrachium rosenbergii was revealed using various techniques, including conventional histology, histochemistry, scanning electron microscopy, and X-ray tomography. The results showed the diversity of cells in the AnG and the detailed organization of the labyrinth\'s tubule into four radiated areas from the central to peripheral zones. The study also demonstrated the expression of some vertebrate kidney-associated homolog genes, aquaporin (AQP), solute carrier family 22 (SLC-22), nephrin, and uromodulin, in the AnG by qPCR. The result of in situ hybridization further showed the localization of SLC-22 and AQP transcript in the bladder and labyrinth\'s epithelium, specifically in regions 2, 3, and 4. Additionally, the study revealed neuropeptide expressions in the AnG by qPCR and in situ hybridization, i.e., crustacean hyperglycemic hormone (CHH) and molt inhibiting hormone (MIH), implying that the AnG may have a role in hormone production. Moreover, male and female prawns exhibited different levels of AQP, SLC-22, nephrin, and CHH expressions during the premolt and intermolt stages, suggesting a crucial role relevant to the molting stages. In conclusion, this study clarified the complex structure of the AnG in M. rosenbergii and demonstrated for the first time the expression of vertebrate kidney-associated genes and the possible endocrine role of the AnG. Further investigation is needed to clarify the role of these genes, particularly during ecdysis. The implications of these findings could significantly advance our understanding of the AnG in decapod crustaceans.
摘要：
在这项研究中,使用各种技术揭示了大型淡水对虾罗氏沼虾中AnG的复杂组织，包括常规组织学,组织化学,扫描电子显微镜，和X射线断层扫描.结果表明，AnG中细胞的多样性以及迷宫小管从中心到外围区域的四个辐射区的详细组织。该研究还证明了一些脊椎动物肾脏相关同源基因的表达，水通道蛋白(AQP),溶质载体家族22（SLC-22），nephrin,和尿调蛋白，通过qPCR在AnG中。原位杂交结果进一步显示SLC-22和AQP转录本在膀胱和迷路上皮中的定位,特别是在区域2、3和4中。此外,该研究通过qPCR和原位杂交揭示了AnG中神经肽的表达，即，甲壳类高血糖激素（CHH）和蜕皮抑制激素（MIH），暗示AnG可能在激素产生中起作用。此外，雄性和雌性对虾表现出不同水平的AQP，SLC-22,nephrin,和CHH在预蜕皮和间蜕皮阶段的表达，表明了与蜕皮阶段相关的关键作用。总之,这项研究阐明了罗森伯氏菌中AnG的复杂结构，并首次证明了脊椎动物肾脏相关基因的表达以及AnG可能的内分泌作用。需要进一步的研究来阐明这些基因的作用，特别是在蜕皮期间。这些发现的含义可以显着提高我们对足类甲壳类动物中AnG的理解。
