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Abstract：
Efficiently mitigating and managing environmental pollution caused by the improper disposal of dyes and effluents from the textile industry is of great importance. This study evaluated the effectiveness of Streptomyces albidoflavus 3MGH in decolorizing and degrading three different azo dyes, namely Reactive Orange 122 (RO 122), Direct Blue 15 (DB 15), and Direct Black 38 (DB 38). Various analytical techniques, such as Fourier Transform Infrared (FTIR) spectroscopy, High-Performance Liquid Chromatography (HPLC), and Gas Chromatography-Mass Spectrometry (GC-MS) were used to analyze the degraded byproducts of the dyes. S. albidoflavus 3MGH demonstrated a strong capability to decolorize RO 122, DB 15, and DB 38, achieving up to 60.74%, 61.38%, and 53.43% decolorization within 5 days at a concentration of 0.3 g/L, respectively. The optimal conditions for the maximum decolorization of these azo dyes were found to be a temperature of 35 °C, a pH of 6, sucrose as a carbon source, and beef extract as a nitrogen source. Additionally, after optimization of the decolorization process, treatment with S. albidoflavus 3MGH resulted in significant reductions of 94.4%, 86.3%, and 68.2% in the total organic carbon of RO 122, DB 15, and DB 38, respectively. After the treatment process, we found the specific activity of the laccase enzyme, one of the mediating enzymes of the degradation mechanism, to be 5.96 U/mg. FT-IR spectroscopy analysis of the degraded metabolites showed specific changes and shifts in peaks compared to the control samples. GC-MS analysis revealed the presence of metabolites such as benzene, biphenyl, and naphthalene derivatives. Overall, this study demonstrated the potential of S. albidoflavus 3MGH for the effective decolorization and degradation of different azo dyes. The findings were validated through various analytical techniques, shedding light on the biodegradation mechanism employed by this strain.
摘要：
有效地减轻和管理由纺织工业的染料和废水的不当处置引起的环境污染非常重要。本研究评估了黄链霉菌3MGH对三种不同的偶氮染料的脱色和降解效果，即反应橙122(RO122)，直接蓝15(DB15)，和直接黑38（DB38）。各种分析技术，如傅里叶变换红外（FTIR）光谱，高效液相色谱法(HPLC),使用气相色谱-质谱（GC-MS）分析染料的降解副产物。S.albidofavus3MGH表现出强大的脱色能力RO122，DB15和DB38，达到60.74％，61.38%，在浓度为0.3g/L的情况下，5天内脱色53.43%，分别。发现这些偶氮染料最大脱色的最佳条件是35°C的温度，pH值为6，蔗糖作为碳源，和牛肉提取物作为氮源。此外,脱色工艺优化后，用S.albidofavus3MGH治疗导致94.4％的显着减少，86.3%,分别占RO122、DB15和DB38总有机碳的68.2%。经过治疗,我们发现了漆酶的特定活性，降解机制的介导酶之一，为5.96U/mg。与对照样品相比，降解代谢物的FT-IR光谱分析显示出峰的特定变化和位移。GC-MS分析显示代谢物如苯的存在，联苯,和萘衍生物。总的来说,这项研究证明了S.albidofavus3MGH对不同偶氮染料的有效脱色和降解的潜力。这些发现通过各种分析技术得到了验证，阐明了该菌株采用的生物降解机制。
