Abstract：
OBJECTIVE: We assessed the feasibility of storing sera in primary gel separator tube over medium-term for retrospective serological tests to facilitate investigation of intra-uterine infection.
METHODS: 120 residual serum samples, consisting of 30 positive samples each for rubella, cytomegalovirus, parvovirus B19 and varicella zoster IgG were aliquoted into secondary propylene tubes and stored together with the original primary tubes at -20°C for 1 year. The serum was subsequently retested to compare results from both storage methods.
RESULTS: Haemolysis was observed in 49.2% of serum stored in the primary tubes. However, there was no difference in both the qualitative and quantitative results after storage of serum samples in either receptacle.
CONCLUSIONS: Sera can be stored in primary blood tube for up to 1 year without affecting serological results. For laboratories with adequate freezer space to store samples in primary blood tubes, this would streamline workflow saving manpower and time, avoid mislabelling of aliquots, reduce consumable costs and prevent unnecessary biohazard exposures.
METHODS: 120 residual serum samples, consisting of 30 positive samples each for rubella, cytomegalovirus, parvovirus B19 and varicella zoster IgG were aliquoted into secondary propylene tubes and stored together with the original primary tubes at -20°C for 1 year. The serum was subsequently retested to compare results from both storage methods.
RESULTS: Haemolysis was observed in 49.2% of serum stored in the primary tubes. However, there was no difference in both the qualitative and quantitative results after storage of serum samples in either receptacle.
CONCLUSIONS: Sera can be stored in primary blood tube for up to 1 year without affecting serological results. For laboratories with adequate freezer space to store samples in primary blood tubes, this would streamline workflow saving manpower and time, avoid mislabelling of aliquots, reduce consumable costs and prevent unnecessary biohazard exposures.
摘要：
目的：我们评估了在中期内将血清储存在一级凝胶分离管中进行回顾性血清学检查的可行性，以促进宫内感染的调查。
方法：120份残余血清样本，由30份风疹阳性样本组成，巨细胞病毒,将细小病毒B19和水痘带状疱疹IgG等分到二级丙烯管中，并与原始一级管一起在-20°C下储存1年。随后重新测试血清以比较两种储存方法的结果。
结果：在保存在初级管中的49.2％血清中观察到溶血。然而,血清样品在任一容器中储存后，定性和定量结果均无差异。
结论：血清可以在初级血管中储存长达1年，而不会影响血清学结果。对于有足够冷冻空间将样本储存在初级血液管中的实验室，这将简化工作流程，节省人力和时间，避免错误标记等分试样，降低消费品成本，防止不必要的生物危害暴露。
方法：120份残余血清样本，由30份风疹阳性样本组成，巨细胞病毒,将细小病毒B19和水痘带状疱疹IgG等分到二级丙烯管中，并与原始一级管一起在-20°C下储存1年。随后重新测试血清以比较两种储存方法的结果。
结果：在保存在初级管中的49.2％血清中观察到溶血。然而,血清样品在任一容器中储存后，定性和定量结果均无差异。
结论：血清可以在初级血管中储存长达1年，而不会影响血清学结果。对于有足够冷冻空间将样本储存在初级血液管中的实验室，这将简化工作流程，节省人力和时间，避免错误标记等分试样，降低消费品成本，防止不必要的生物危害暴露。
