Abstract：
Organophosphorus pesticides (OPPs) are important chemical stressors in aquatic ecosystems, and they attract increasing more attentions recently. However, the impacts of different OPPs on carbon cycling remain unclear, particularly for those functional-yet-uncultivable microbes. This study investigated the change in lake aquatic microbial communities in the presence of dichlorvos, monocrotophos, omethoate and parathion. All OPPs significantly inhibited biomass (p < 0.05) and the expression of carbon cycle-related cbbLG gene (p < 0.01), and altered aquatic microbial community structure, interaction, and assembly. Variance partitioning analysis showed a stronger impact of pesticide type on microbial biomass and community structure, where pesticide concentration played more significant roles in carbon cycling. From analysis of cbbLG gene and PICRUSt2, Luteolibacter and Verrucomicrobiaceae assimilated inorganic carbon through Wood-Ljungdahl pathway, whereas it was Calvin-Benson-Bassham cycle for Cyanobium PCC-6307. This work provides a deeper insight into the behavior and mechanisms of microbial community change in aquatic system in response to OPPs, and explicitly unravels the impacts of OPPs on their carbon-cycling functions.
摘要：
有机磷农药(OPPs)是水生生态系统中重要的化学应激源,最近它们吸引了越来越多的关注。然而,不同OPPs对碳循环的影响尚不清楚，特别是那些功能性但无法培养的微生物。这项研究调查了在敌敌畏存在下湖泊水生微生物群落的变化，久效磷,氧乐果和对硫磷。所有OPP均显著抑制生物量(p<0.05)和碳循环相关cbbLG基因表达(p<0.01),改变了水生微生物群落结构，互动,和组装。方差分配分析表明，农药类型对微生物生物量和群落结构的影响更大。其中农药浓度在碳循环中起着更重要的作用。通过Wood-Ljungdahl途径分析cbbLG基因和PICRUSt2，木脂杆菌和麻黄菌科同化的无机碳，而CyanobiumPCC-6307是Calvin-Benson-Bassham循环。这项工作提供了一个更深入的见解的行为和机制的微生物群落变化在水生生物多样性的反应，并明确揭示了OPP对其碳循环功能的影响。
