PDF(Pubmed)
Abstract：
Viral infectivity depends on multiple factors. Recent studies showed that the interaction between viral RNAs and endogenous microRNAs (miRNAs) regulates viral infectivity; viral RNAs function as a sponge of endogenous miRNAs and result in upregulation of its original target genes, while endogenous miRNAs target viral RNAs directly and result in repression of viral gene expression. In this study, we analyzed the possible interaction between parainfluenza virus RNA and endogenous miRNAs in human and mouse lungs. We showed that the parainfluenza virus can form base pairs with human miRNAs abundantly than mouse miRNAs. Furthermore, we analyzed that the sponge effect of endogenous miRNAs on viral RNAs may induce the upregulation of transcription regulatory factors. Then, we performed RNA-sequence analysis and observed the upregulation of transcription regulatory factors in the early stages of parainfluenza virus infection. Our studies showed how the differential expression of endogenous miRNAs in lungs could contribute to respiratory virus infection and species- or tissue-specific mechanisms and common mechanisms could be conserved in humans and mice and regulated by miRNAs during viral infection.
摘要：
病毒的感染性取决于多种因素。最近的研究表明，病毒RNA和内源性microRNA（miRNA）之间的相互作用调节病毒的感染性；病毒RNA作为内源性miRNA的海绵，并导致其原始靶基因的上调，而内源性miRNA直接靶向病毒RNA并导致病毒基因表达的抑制。在这项研究中,我们分析了副流感病毒RNA与人和小鼠肺中内源性miRNAs之间可能的相互作用.我们表明副流感病毒可以与人miRNA形成比小鼠miRNA丰富的碱基对。此外，我们分析了内源性miRNAs对病毒RNAs的海绵效应可能诱导转录调控因子的上调。然后，我们进行了RNA序列分析,观察了副流感病毒感染早期转录调节因子的上调.我们的研究表明，内源性miRNAs在肺中的差异表达如何导致呼吸道病毒感染，物种或组织特异性机制和共同机制在人类和小鼠中可以被保留，并在病毒感染期间由miRNAs调节。
