Abstract：
Diphyllobothriosis, a fish-borne zoonosis in South America, is mainly caused by the Pacific broad tapeworm Adenocephalus pacificus Nybelin, 1931, a parasite of considerable concern in fishery resources due to its impact on public health. A new diphyllobothrid, Diphyllobothrium sprakeri Hernández-Orts et al. Parasites Vectors 14:219, 2021, was recently described from sea lions from the Pacific Coast, but marine fish acting as intermediate hosts are unknown. The objective of this study was to confirm the presence of plerocercoid larvae of Diphyllobothriidae Lühe, 1910 (Cestoda: Diphyllobothriidea) in nine fish species of commercial importance in Peru. Of a total of 6999 fish (5861 Engraulis ringens, 853 Sciaena deliciosa, 6 Sciaena callaensis, 171 Scomber japonicus, 40 Trachurus murphyi, 40 Ariopsis seemanni, 18 Merluccius peruanus, 5 Sarda chiliensis, and 5 Coryphaena hippurus), 183 were infected with plerocercoid larvae, representing a total prevalence of 2.61% and a mean intensity of 3.2. Based on mtDNA cox1 sequences of 43 plerocercoids, a phylogenetic analysis revealed that 41 belong to A. pacificus and two to D. sprakeri. These findings are first molecular data for D. sprakeri larvae, and the infections of E. ringens and T. murphyi by plerocercoid larvae represent the first records of intermediate/paratenic hosts for this species. Hence, the findings of the current study enhance our understanding of the presence of diphyllobothriid species in commercial fish from the Southeastern Pacific Ocean and their potential impact on seafood safety for local human populations.
摘要：
双叶病,南美洲的鱼类动物共患病，主要是由太平洋广泛的a虫引起的，1931年，由于其对公共卫生的影响，在渔业资源中引起了相当大的关注。一种新的双叶虫，DiphyllobothriumsprakeriHernández-Ortsetal.寄生虫Vectors14：219，2021，最近被描述为来自太平洋海岸的海狮，但是作为中间宿主的海鱼是未知的。这项研究的目的是确认DiphyllobothriidaeLühe的plerocercoid幼虫的存在，1910年（Cestoda：Diphyllobothriidea）在秘鲁具有商业重要性的9种鱼类中。在总共6999条鱼中(5861条Engraulisringens，853Sciaenadeliciosa,：171刺槐，40.Murphyi，40Ariopsisseemanni,18PeruanusMerluccius,5萨达辣椒，和5个河豚)，183例感染了骨灰质幼虫，总患病率为2.61%，平均强度为3.2。基于43种植物的mtDNAcox1序列，系统发育分析表明，有41个属于A.pacificus，两个属于D.sprakeri。这些发现是D.sprakeri幼虫的第一个分子数据，plerocercoid幼虫对E.ringens和T.murphyi的感染代表了该物种的中间/旁宿主的第一个记录。因此，本研究的结果增强了我们对东南太平洋商业鱼类中二苯基关节炎物种的存在及其对当地人群海鲜安全的潜在影响的理解。
