PDF(Pubmed)
Abstract：
UNASSIGNED: Forest musk deer (FMD, Moschus Berezovskii) is a critically endangered species world-widely, the death of which can be caused by pulmonary disease in the farm. Pulmonary fibrosis (PF) was a huge threat to the health and survival of captive FMD. MicroRNAs (miRNAs) and messenger RNAs (mRNAs) have been involved in the regulation of immune genes and disease development. However, the regulatory profiles of mRNAs and miRNAs involved in immune regulation of FMD are unclear.
UNASSIGNED: In this study, mRNA-seq and miRNA-seq in blood were performed to constructed coexpression regulatory networks between PF and healthy groups of FMD. The hub immune- and apoptosis-related genes in the PF blood of FMD were explored through Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Further, protein-protein interaction (PPI) network of immune-associated and apoptosis-associated key signaling pathways were constructed based on mRNA-miRNA in the PF blood of the FMD. Immune hub DEGs and immune hub DEmiRNAs were selected for experimental verification using RT-qPCR.
UNASSIGNED: A total of 2744 differentially expressed genes (DEGs) and 356 differentially expressed miRNAs (DEmiRNAs) were identified in the PF blood group compared to the healthy blood group. Among them, 42 DEmiRNAs were negatively correlated with 20 immune DEGs from a total of 57 correlations. The DEGs were significantly associated with pathways related to CD molecules, immune disease, immune system, cytokine receptors, T cell receptor signaling pathway, Th1 and Th2 cell differentiation, cytokine-cytokine receptor interaction, intestinal immune network for IgA production, and NOD-like receptor signaling pathway. There were 240 immune-related DEGs, in which 186 immune-related DEGs were up-regulated and 54 immune-related DEGs were down-regulated. In the protein-protein interaction (PPI) analysis of immune-related signaling pathway, TYK2, TLR2, TLR4, IL18, CSF1, CXCL13, LCK, ITGB2, PIK3CB, HCK, CD40, CD86, CCL3, CCR7, IL2RA, TLR3, and IL4R were identified as the hub immune genes. The mRNA-miRNA coregulation analysis showed that let-7d, miR-324-3p, miR-760, miR-185, miR-149, miR-149-5p, and miR-1842-5p are key miRNAs that target DEGs involved in immune disease, immune system and immunoregulation.
UNASSIGNED: The development and occurrence of PF were significantly influenced by the immune-related and apoptosis-related genes present in PF blood. mRNAs and miRNAs associated with the development and occurrence of PF in the FMD.
UNASSIGNED: In this study, mRNA-seq and miRNA-seq in blood were performed to constructed coexpression regulatory networks between PF and healthy groups of FMD. The hub immune- and apoptosis-related genes in the PF blood of FMD were explored through Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Further, protein-protein interaction (PPI) network of immune-associated and apoptosis-associated key signaling pathways were constructed based on mRNA-miRNA in the PF blood of the FMD. Immune hub DEGs and immune hub DEmiRNAs were selected for experimental verification using RT-qPCR.
UNASSIGNED: A total of 2744 differentially expressed genes (DEGs) and 356 differentially expressed miRNAs (DEmiRNAs) were identified in the PF blood group compared to the healthy blood group. Among them, 42 DEmiRNAs were negatively correlated with 20 immune DEGs from a total of 57 correlations. The DEGs were significantly associated with pathways related to CD molecules, immune disease, immune system, cytokine receptors, T cell receptor signaling pathway, Th1 and Th2 cell differentiation, cytokine-cytokine receptor interaction, intestinal immune network for IgA production, and NOD-like receptor signaling pathway. There were 240 immune-related DEGs, in which 186 immune-related DEGs were up-regulated and 54 immune-related DEGs were down-regulated. In the protein-protein interaction (PPI) analysis of immune-related signaling pathway, TYK2, TLR2, TLR4, IL18, CSF1, CXCL13, LCK, ITGB2, PIK3CB, HCK, CD40, CD86, CCL3, CCR7, IL2RA, TLR3, and IL4R were identified as the hub immune genes. The mRNA-miRNA coregulation analysis showed that let-7d, miR-324-3p, miR-760, miR-185, miR-149, miR-149-5p, and miR-1842-5p are key miRNAs that target DEGs involved in immune disease, immune system and immunoregulation.
UNASSIGNED: The development and occurrence of PF were significantly influenced by the immune-related and apoptosis-related genes present in PF blood. mRNAs and miRNAs associated with the development and occurrence of PF in the FMD.
摘要：
■森林麝香鹿（口蹄疫，MoschusBerezovskii）是世界范围内极度濒危的物种，其死亡可能是由农场的肺部疾病引起的。肺纤维化（PF）对圈养FMD的健康和生存构成巨大威胁。MicroRNAs(miRNAs)和信使RNAs(mRNAs)参与了免疫基因的调控和疾病的发生发展。然而,参与FMD免疫调节的mRNA和miRNA的调控谱尚不清楚.
■在这项研究中，在血液中进行mRNA-seq和miRNA-seq以构建PF和健康FMD组之间的共表达调节网络。通过基因本体论（GO）和京都基因和基因组百科全书（KEGG）富集分析，探索了FMDPF血液中的枢纽免疫和凋亡相关基因。Further,基于FMDPF血液中的mRNA-miRNA，构建了免疫相关和凋亡相关关键信号通路的蛋白-蛋白相互作用（PPI）网络。选择免疫枢纽DEG和免疫枢纽DEmiRNA用于使用RT-qPCR的实验验证。
■与健康血型相比,在PF血型中鉴定出总共2744个差异表达基因(DEG)和356个差异表达miRNA(DEmiRNA)。其中,在总共57个相关性中，42个DEmiRNAs与20个免疫DEGs呈负相关。DEGs与CD分子相关的通路显著相关,免疫性疾病，免疫系统,细胞因子受体,T细胞受体信号通路,Th1和Th2细胞分化，细胞因子-细胞因子受体相互作用，产生IgA的肠道免疫网络，和NOD样受体信号通路。有240个免疫相关的DEG，其中186个免疫相关的DEGs上调,54个免疫相关的DEGs下调.在免疫相关信号通路的蛋白质-蛋白质相互作用（PPI）分析中，TYK2、TLR2、TLR4、IL18、CSF1、CXCL13、LCK、ITGB2,PIK3CB,HCK,CD40,CD86,CCL3,CCR7,IL2RA,TLR3和IL4R被鉴定为中枢免疫基因。mRNA-miRNA共调控分析表明，let-7d，miR-324-3p,miR-760,miR-185,miR-149,miR-149-5p,miR-1842-5p是靶向参与免疫性疾病的DEG的关键miRNA，免疫系统和免疫调节。
■PF血液中存在的免疫相关和凋亡相关基因显着影响PF的发生和发生。mRNA和miRNA与FMD中PF的发生发展有关。
■在这项研究中，在血液中进行mRNA-seq和miRNA-seq以构建PF和健康FMD组之间的共表达调节网络。通过基因本体论（GO）和京都基因和基因组百科全书（KEGG）富集分析，探索了FMDPF血液中的枢纽免疫和凋亡相关基因。Further,基于FMDPF血液中的mRNA-miRNA，构建了免疫相关和凋亡相关关键信号通路的蛋白-蛋白相互作用（PPI）网络。选择免疫枢纽DEG和免疫枢纽DEmiRNA用于使用RT-qPCR的实验验证。
■与健康血型相比,在PF血型中鉴定出总共2744个差异表达基因(DEG)和356个差异表达miRNA(DEmiRNA)。其中,在总共57个相关性中，42个DEmiRNAs与20个免疫DEGs呈负相关。DEGs与CD分子相关的通路显著相关,免疫性疾病，免疫系统,细胞因子受体,T细胞受体信号通路,Th1和Th2细胞分化，细胞因子-细胞因子受体相互作用，产生IgA的肠道免疫网络，和NOD样受体信号通路。有240个免疫相关的DEG，其中186个免疫相关的DEGs上调,54个免疫相关的DEGs下调.在免疫相关信号通路的蛋白质-蛋白质相互作用（PPI）分析中，TYK2、TLR2、TLR4、IL18、CSF1、CXCL13、LCK、ITGB2,PIK3CB,HCK,CD40,CD86,CCL3,CCR7,IL2RA,TLR3和IL4R被鉴定为中枢免疫基因。mRNA-miRNA共调控分析表明，let-7d，miR-324-3p,miR-760,miR-185,miR-149,miR-149-5p,miR-1842-5p是靶向参与免疫性疾病的DEG的关键miRNA，免疫系统和免疫调节。
■PF血液中存在的免疫相关和凋亡相关基因显着影响PF的发生和发生。mRNA和miRNA与FMD中PF的发生发展有关。
