PDF(Pubmed)
Abstract：
UNASSIGNED: Microorganisms in biofilms are particularly difficult to control because of their increased survival and antibiotic resistance. Allicin and domiphen were employed to inhibit the microbial growth and biofilm formation of Staphylococcus aureus, Escherichia coli, and Candida albicans strains.
UNASSIGNED: Broth microdilution method and checkerboard assay were conducted to determine the efficacy of allicin combined with domiphen against S. aureus, E. coli, and C. albicans. Microbial biofilm formation was measured using the crystal violet staining method and fluorescence microscopy. And the total viable count of the biofilm cells on material surface after the treatment with antimicrobial reagents was calculated with the plate count technique.
UNASSIGNED: The two drugs showed synergistic effects against the pathogens with a fractional bactericidal concentration of less than 0.38. The combination of 64 μg/mL allicin with 1 μg/mL domiphen dispersed over 50% of the biofilm mass of S. aureus, E. coli, and C. albicans. In addition, the drug combination reduced the total viable counts of E. coli and C. albicans biofilm cells on stainless steel and polyethylene surfaces by more than 102 CFU/mL.
UNASSIGNED: The combination of allicin and domiphen is an effective strategy for efficiently decreasing biofilms formation on various industrial materials surfaces.
UNASSIGNED: Broth microdilution method and checkerboard assay were conducted to determine the efficacy of allicin combined with domiphen against S. aureus, E. coli, and C. albicans. Microbial biofilm formation was measured using the crystal violet staining method and fluorescence microscopy. And the total viable count of the biofilm cells on material surface after the treatment with antimicrobial reagents was calculated with the plate count technique.
UNASSIGNED: The two drugs showed synergistic effects against the pathogens with a fractional bactericidal concentration of less than 0.38. The combination of 64 μg/mL allicin with 1 μg/mL domiphen dispersed over 50% of the biofilm mass of S. aureus, E. coli, and C. albicans. In addition, the drug combination reduced the total viable counts of E. coli and C. albicans biofilm cells on stainless steel and polyethylene surfaces by more than 102 CFU/mL.
UNASSIGNED: The combination of allicin and domiphen is an effective strategy for efficiently decreasing biofilms formation on various industrial materials surfaces.
摘要：
■生物膜中的微生物由于其增加的存活率和抗生素抗性而特别难以控制。大蒜素和domiphen用于抑制金黄色葡萄球菌的微生物生长和生物膜形成，大肠杆菌,和白色念珠菌菌株。
■肉汤微量稀释法和棋盘法测定大蒜素联合多米芬对金黄色葡萄球菌的疗效，大肠杆菌，还有白色念珠菌.使用结晶紫染色法和荧光显微镜测量微生物生物膜的形成。用平板计数技术计算用抗菌试剂处理后材料表面生物膜细胞的总活菌数。
■两种药物对病原体表现出协同作用，杀菌浓度小于0.38。64μg/mL大蒜素与1μg/mLdomiphen的组合分散在金黄色葡萄球菌的生物膜质量的50％，大肠杆菌，还有白色念珠菌.此外,该药物组合使不锈钢和聚乙烯表面上的大肠杆菌和白色念珠菌生物膜细胞的总活菌计数减少超过102CFU/mL。
■大蒜素和domiphen的组合是有效减少各种工业材料表面上生物膜形成的有效策略。
■肉汤微量稀释法和棋盘法测定大蒜素联合多米芬对金黄色葡萄球菌的疗效，大肠杆菌，还有白色念珠菌.使用结晶紫染色法和荧光显微镜测量微生物生物膜的形成。用平板计数技术计算用抗菌试剂处理后材料表面生物膜细胞的总活菌数。
■两种药物对病原体表现出协同作用，杀菌浓度小于0.38。64μg/mL大蒜素与1μg/mLdomiphen的组合分散在金黄色葡萄球菌的生物膜质量的50％，大肠杆菌，还有白色念珠菌.此外,该药物组合使不锈钢和聚乙烯表面上的大肠杆菌和白色念珠菌生物膜细胞的总活菌计数减少超过102CFU/mL。
■大蒜素和domiphen的组合是有效减少各种工业材料表面上生物膜形成的有效策略。
