PDF(Pubmed)
Abstract：
Excessive fat deposition due to impaired fat metabolism in chickens is a major problem in the poultry industry. Nutritional interventions are effective solutions, but current options are limited. A safe phytochemical, rutin, has shown positive effects in animals, but its effect on lipid metabolism in poultry remains unknown. Hence, this study is to investigate the effects of rutin on egg quality, serum biochemistry, fat deposition, lipid peroxidation and hepatic lipid metabolism in post-peak laying hens. A total of 360 Taihang laying hens (49-week-old) were randomly divided into five groups and fed a basal diet (control group, 0%) and a basal diet supplemented with 300 (0.03%), 600 (0.06%), 900 (0.09%), and 1,200 (0.12%) mg rutin/kg feed, respectively. The results showed that eggshell strength was significantly (p < 0.05) higher in the dietary rutin groups, whereas yolk percentage (p < 0.05), total cholesterol (TC) (p < 0.01) and yolk fat ratio (p < 0.01) decreased linearly (p < 0.05) in the dietary rutin groups. Importantly, dietary rutin reduced serum triglyceride (TG) and TC levels, decreased abdominal lipid deposition and liver index (p < 0.05), and which concomitantly decreased hepatic lipid (TG, TC, and free fatty acid) accumulation (p < 0.05). An increase (p < 0.05) in total antioxidant capacity and superoxide dismutase activity and a decrease (p < 0.05) in malondialdehyde levels were also found. At the same time, the activities of hepatic lipase, acetyl-CoA carboxylase and malic enzyme in the liver were decreased (p < 0.05). Dietary rutin also increased (p < 0.05) the expression of fatty acid oxidation-related genes (carnitine palmitoyl transferase 1, peroxisome proliferator-activated receptor α, farnesoid X receptor). Additionally, it decreased fatty acid synthesis genes (sterol regulatory element binding protein-1c, acetyl-CoA carboxylase α, stearoyl-CoA desaturase 1) (p < 0.05). In conclusion, the addition of rutin (0.06-0.12%) to the diet improved the fat metabolism and increased liver antioxidant capacity in post-peak laying hens, and these positive changes improved egg quality to some extent.
摘要：
由于鸡的脂肪代谢受损而导致的过度脂肪沉积是家禽业中的主要问题。营养干预是有效的解决方案，但目前的选择是有限的。一种安全的植物化学物质,芦丁,对动物有积极的影响，但其对家禽脂质代谢的影响尚不清楚。因此，本研究旨在探讨芦丁对鸡蛋品质的影响,血清生物化学,脂肪沉积,峰后蛋鸡的脂质过氧化和肝脏脂质代谢。将360只太行蛋鸡(49周龄)随机分为5组,饲喂基础日粮(对照组,0％）和补充300（0.03％）的基础饮食，600（0.06％），900（0.09%），和1200(0.12%)毫克芦丁/公斤饲料，分别。结果表明,饲粮芦丁组蛋壳强度显著(p<0.05)提高,而蛋黄百分比(p<0.05)，总胆固醇（TC）（p<0.01）和蛋黄脂肪比（p<0.01）线性降低（p<0.05）。重要的是,饲粮芦丁降低血清甘油三酯（TG）和TC水平，降低腹部脂质沉积和肝脏指数（p<0.05），并同时降低肝脂质(TG，TC,和游离脂肪酸)积累(p<0.05)。还发现总抗氧化能力和超氧化物歧化酶活性增加（p<0.05），丙二醛水平降低（p<0.05）。同时,肝脂肪酶的活性，肝脏中乙酰辅酶A羧化酶和苹果酸酶均降低(p<0.05)。日粮芦丁也增加（p<0.05）脂肪酸氧化相关基因的表达（肉碱棕榈酰转移酶1，过氧化物酶体增殖物激活受体α，法尼醇X受体)。此外,它降低了脂肪酸合成基因(甾醇调节元件结合蛋白-1c，乙酰辅酶A羧化酶α,硬脂酰辅酶A去饱和酶1)(p<0.05)。总之,日粮中添加芦丁(0.06-0.12%)改善了高峰后蛋鸡的脂肪代谢和肝脏抗氧化能力,这些积极的变化在一定程度上改善了鸡蛋的品质。
