METHODS: A cross-sectional analytical study was conducted in the infertility clinic of a tertiary care hospital. A total of 106 infertile males with abnormal semen analysis as per the WHO criteria (2010) were enrolled in the study. After routine semen analysis, SDF was studied using the comet assay. The serum fasting lipid profile was analyzed using the spectrophotometric kit in the autoanalyzer. The relationship of SDF with serum lipid profile parameters was analyzed.
RESULTS: Out of 106 infertile men, 52% (n = 55) had severe SDF. A modest positive correlation was observed between SDF (percentage of DNA in comet tail) and serum lipid values (serum TG, serum LDL, and serum VLDL).
CONCLUSIONS: Our study is novel in its research on the correlation between SDF and serum lipid values. Based on the findings of our study, it can be concluded that a significant level of SDF was observed in men with high levels of serum TG, LDL, and VLDL. This provokes a potential relationship between sperm DNA integrity and serum lipid profile, which warrants further research.
方法:在三级保健医院的不孕症诊所进行了一项横断面分析研究。根据WHO标准(2010年),共有106名不育男性精液分析异常。常规精液分析后,使用彗星测定法研究SDF。使用自动分析仪中的分光光度试剂盒分析血清空腹血脂谱。分析SDF与血脂参数的关系。
结果:在106名不育男性中,52%(n=55)患有严重的SDF。在SDF(彗星尾巴中DNA的百分比)和血清脂质值(血清TG,血清LDL,和血清VLDL)。
结论:我们的研究在研究SDF与血脂值之间的相关性方面是新颖的。根据我们的研究结果,可以得出结论,在血清TG水平高的男性中观察到显著水平的SDF,LDL,VLDL。这引发了精子DNA完整性和血清脂质分布之间的潜在关系,这值得进一步研究。