Abstract：
Atractylodes macrocephala Koidz, a traditional Chinese medicine, contains atractylenolide I (ATR-I), which has potential anticancer, anti-inflammatory, and immune-modulating properties. This study evaluated the therapeutic potential of ATR-I for indomethacin (IND)-induced gastric mucosal lesions and its underlying mechanisms. Noticeable improvements were observed in the histological morphology and ultrastructures of the rat gastric mucosa after ATR-I treatment. There was improved blood flow, a significant decrease in the expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-1β, and IL-18, and a marked increase in prostaglandin E2 (PGE2) expression in ATR-I-treated rats. Furthermore, there was a significant decrease in the mRNA and protein expression levels of NOD-like receptor thermal protein domain associated protein 3 (NLRP3), apoptosis-associated speck-like protein (ASC), cysteinyl aspartate specific proteinase-1 (caspase-1), and nuclear factor-κB (NF-κB) in rats treated with ATR-I. The results show that ATR-I inhibits the NLRP3 inflammasome signaling pathway and effectively alleviates local inflammation, thereby improving the therapeutic outcomes against IND-induced gastric ulcers in rats.
摘要：
白术，中药，含有白术内酯I（ATR-I），具有潜在的抗癌作用，抗炎,和免疫调节特性。这项研究评估了ATR-I对吲哚美辛（IND）诱导的胃粘膜病变的治疗潜力及其潜在机制。ATR-I治疗后，大鼠胃粘膜的组织学形态和超微结构得到了显着改善。血流改善了,肿瘤坏死因子-α（TNF-α）的表达显着降低，白细胞介素-6（IL-6），IL-1β,和IL-18，并且在ATR-I处理的大鼠中前列腺素E2（PGE2）表达显着增加。此外，NOD样受体热蛋白结构域相关蛋白3（NLRP3）的mRNA和蛋白表达水平显著下降，凋亡相关斑点样蛋白(ASC)，半胱氨酰天冬氨酸特异性蛋白酶-1（caspase-1），ATR-I治疗大鼠的核因子-κB（NF-κB）结果表明,ATR-I抑制NLRP3炎症小体信号通路,有效缓解局部炎症,从而改善大鼠对IND诱导的胃溃疡的治疗效果。
