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Abstract：
Currently, the increasing pollution of the environment by heavy metals is observed, caused both by natural factors and those related to human activity. They pose a significant threat to human health and life. It is therefore important to find an effective way of protecting organisms from their adverse effects. One potential product showing a protective effect is green tea. It has been shown that EGCG, which is found in large amounts in green tea, has strong antioxidant properties and can therefore protect cells from the adverse effects of heavy metals. Therefore, the aim of the study was to investigate the effect of EGCG on cells exposed to Cd. In the study, CHO-K1 cells (Chinese hamster ovary cell line) were treated for 24 h with Cd (5 and 10 µM) and EGCG (0.5 and 1 µM) together or separately. Cell viability, ATP content, total ROS activity, mitochondrial membrane potential and apoptosis potential were determined. The results showed that, in tested concentrations, EGCG enhanced the negative effect of Cd. Further analyses are needed to determine the exact mechanism of action of EGCG due to the small number of publications on the subject and the differences in the results obtained in the research.
摘要：
目前,观察到重金属对环境的污染日益严重，由自然因素和与人类活动有关的因素引起的。它们对人类健康和生命构成重大威胁。因此，重要的是找到一种有效的方法来保护生物体免受其不利影响。一种显示保护作用的潜在产品是绿茶。已经表明EGCG,在绿茶中大量发现，具有很强的抗氧化性能，因此可以保护细胞免受重金属的不利影响。因此,该研究的目的是研究EGCG对暴露于Cd的细胞的影响。在研究中,CHO-K1细胞（中国仓鼠卵巢细胞系）用Cd（5和10µM）和EGCG（0.5和1µM）一起或分别处理24小时。细胞活力，ATP含量,ROS总活性，测定线粒体膜电位和凋亡电位。结果表明,在测试浓度中，EGCG增强了Cd的负效应。由于有关该主题的出版物数量少以及研究中获得的结果差异，需要进一步分析以确定EGCG的确切作用机制。
