PDF(Pubmed)
Abstract：
Sclerotinia stem rot (SSR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating diseases for several major oil-producing crops. Despite its impact, the genetic basis of SSR resistance in plants remains poorly understood. Here, through a genome-wide association study, we identify a key gene, BnaA07. MKK9, that encodes a mitogen-activated protein kinase kinase that confers SSR resistance in oilseed rape. Our functional analyses reveal that BnaA07.MKK9 interacts with BnaC03.MPK3 and BnaC03.MPK6 and phosphorylates them at the TEY activation motif, triggering a signaling cascade that initiates biosynthesis of ethylene, camalexin, and indole glucosinolates, and promotes accumulation of H2O2 and the hypersensitive response, ultimately conferring resistance. Furthermore, variations in the coding sequence of BnaA07.MKK9 alter its kinase activity and improve SSR resistance by ~30% in cultivars carrying the advantageous haplotype. These findings enhance our understanding of SSR resistance and may help engineer novel diversity for future breeding of oilseed rape.
摘要：
菌核病茎腐病(SSR),由坏死菌核病菌引起，是几种主要产油作物最具破坏性的疾病之一。尽管有影响,植物中SSR抗性的遗传基础仍然知之甚少。这里,通过全基因组关联研究，我们确定了一个关键基因，BnaA07.MKK9，编码一种丝裂原活化蛋白激酶激酶，赋予油菜SSR抗性。我们的功能分析显示BnaA07。MKK9与BnaC03相互作用。MPK3和BnaC03。MPK6并在TEY激活基序处磷酸化它们，触发引发乙烯生物合成的信号级联，camalexin,和吲哚芥子油苷，并促进H2O2的积累和过敏反应，最终赋予抵抗。此外，BnaA07编码序列的变异。在携带有利单倍型的品种中，MKK9改变了其激酶活性并将SSR抗性提高了约30％。这些发现增强了我们对SSR抗性的理解，并可能有助于为未来油菜育种设计新的多样性。
