Abstract：
This study tries to fill the knowledge gap regarding differences in the expression of proteins in the meat of European wild boar (Sus scrofa scrofa) and domestic pig (Sus scrofa domestica), considering the impact of thermally induced degradation. We assessed relative protein changes between cooked longissimus thoracis et lumborum (LTL) muscle proteomes by using mass spectrometry, chemometric, label-free proteomic, and bioinformatic tools. Among 30 differentially abundant proteins identified MyHC-2a, ATPs-α, CK-S, ADP/ATPt1, IDH2, and MyBP-C1 were upregulated (x > 1) whereas NEB, γ-ENO and EPSF were downregulated (x < 1) in wild boar. ShinyGO and KEGG database pathway analyses revealed that these proteins are mainly involved in processes related to muscle contraction and various pathways of glucose metabolism and energy production. Protein expression changes could have been caused by the different muscle activity of wild animals in response to prolonged movement associated with foraging for food in the natural environment.
摘要：
这项研究试图填补有关欧洲野猪（Susscrofascrofascrofa）和家猪（Susscrofadomestica）肉中蛋白质表达差异的知识空白，考虑到热诱导降解的影响。我们通过使用质谱法评估了煮熟的胸腰长（LTL）肌肉蛋白质组之间的相对蛋白质变化，化学计量学,无标记蛋白质组学，和生物信息学工具。在鉴定出MyHC-2a的30种差异丰富的蛋白质中，ATPs-α,CK-S,ADP/ATPt1、IDH2和MyBP-C1上调(x>1)，而NEB，γ-ENO和EPSF在野猪中下调(x<1)。ShinyGO和KEGG数据库途径分析显示，这些蛋白质主要参与与肌肉收缩相关的过程以及葡萄糖代谢和能量产生的各种途径。蛋白质表达变化可能是由野生动物的不同肌肉活动引起的，以响应与在自然环境中觅食相关的长时间运动。
