Abstract：
For sessile intertidal organisms, periods of low tide impose both cellular and physiological challenges that can determine bathymetric distribution. To understand how intertidal location influences the cellular response of the bivalve Perumytilus purpuratus during the tidal cycle (immersion-emersion-immersion), specimens from the upper intertidal (UI) and lower intertidal (LI) of bathymetric distribution were sampled every 2 h over a 10-h period during a summer tidal cycle. Parallelly, organisms from the UI and LI were reciprocally transplanted and sampled throughout the same tidal cycle. Levels of oxidative damage (lipid peroxidation and protein carbonyls) as well as total antioxidant capacity and total carotenoids were evaluated as cellular responses to variations in environmental conditions throughout the tidal cycle. The results indicate that both the location in the intertidal zone (UI/LI), the level of aerial exposure, and the interaction of both factors are determinants of oxidative levels and total antioxidant capacity of P. purpuratus. Although oxidative damage levels are triggered during the low tide period (aerial exposure), it is the UI specimens that induce higher levels of lipid peroxidation compared to those from the LI, which is consistent with the elevated levels of total antioxidant capacity. On the other hand, organisms from the LI transplanted to the UI increase the levels of lipid peroxidation but not the levels of protein carbonyls, a situation that is also reflected in higher levels of antioxidant response and total carotenoids than those from the UI transplanted to the LI. The bathymetric distribution of P. purpuratus in the intertidal zone implies differentiated responses between organisms of the lower and upper limits, influenced by their life history. A high phenotypic plasticity allows this mussel to adjust its metabolism to respond to abrupt changes in the surrounding environmental conditions.
摘要：
对于固着的潮间带生物，低潮时期施加了细胞和生理挑战，可以确定测深分布。为了了解潮间带位置如何影响双壳类紫癜在潮汐周期（浸入-浸入-浸入）期间的细胞反应，在夏季潮汐周期的10小时内，每2小时采样一次测深分布的上潮间带（UI）和下潮间带（LI）的标本。同样，来自UI和LI的生物体在整个相同的潮汐周期中相互移植和采样。在整个潮汐周期中，氧化损伤（脂质过氧化和蛋白质羰基）以及总抗氧化能力和总类胡萝卜素的水平被评估为细胞对环境条件变化的反应。结果表明，两者都位于潮间带(UI/LI)，空中暴露的水平，两种因素的相互作用是紫癜的氧化水平和总抗氧化能力的决定因素。尽管在低潮期（空中暴露）会触发氧化损伤水平，与LI样本相比，UI样本诱导更高水平的脂质过氧化，这与总抗氧化能力水平的升高是一致的。另一方面,从LI移植到UI的生物体会增加脂质过氧化水平，但不会增加蛋白质羰基水平，这种情况也反映在抗氧化反应和总类胡萝卜素的水平高于移植到LI的UI。潮间带中紫癜的测深分布暗示了下限和上限生物之间的不同反应。受他们生活史的影响。高表型可塑性允许该贻贝调整其代谢以响应周围环境条件的突然变化。
