Abstract：
High temperature is one of the most devastating environmental factors that severely impede plant growth multi-laterally and threatens global food security. Global warming and the predicted steady rise in temperature emphasize the urgent need to improve heat stress resilience of crop plants to meet the growing food demand. Although known for several years, a memory-based mechanism termed \"priming-induced stress tolerance\" or \"acquired stress tolerance\" has gained the attention of the plant science community in recent years. Thermopriming is one such phenomenon that enhances the plant tolerance to subsequent heat stress and promotes growth. The memory of the foregoing mild increase in temperature is captured and retained as dormant signals, which upon exposure to subsequent high temperature aids in mounting a faster, stronger, and sensitized response. Such acquired thermotolerance is more effective than the basal endurance of the plant due to altered molecular regulatory networks. Thereupon, thermopriming can be used as a convenient tool to study and improve plant response to heat stress. In this chapter, the protocol to study thermopriming-mediated short- and long-term acquired thermotolerance is described in detail.
摘要：
高温是最具破坏性的环境因素之一，它从多方面严重阻碍了植物的生长，威胁着全球粮食安全。全球变暖和预测的温度稳步上升强调了迫切需要提高作物植物的热胁迫恢复能力，以满足日益增长的粮食需求。虽然知道了几年，近年来，一种基于记忆的机制被称为“启动诱导的胁迫耐受性”或“获得的胁迫耐受性”，已引起植物科学界的关注。热结晶是一种这样的现象,其增强植物对随后的热胁迫的耐受性并促进生长。上述温度轻度升高的记忆被捕获并保留为休眠信号，暴露于随后的高温有助于更快地安装，更强,和敏感的反应。由于分子调节网络的改变，这种获得的耐热性比植物的基础耐力更有效。于是,热修饰可以作为研究和改善植物对热胁迫反应的一种方便工具。在这一章中,详细介绍了研究热变形介导的短期和长期获得的耐热性的方案.
