Abstract:
Stress can affect different groups of plant metabolites and multiple signaling pathways. Untargeted metabolomics enables the collection of whole-spectrum data for the entire metabolite content present in plant tissues at that point in time. We present a thorough approach for large-scale, untargeted metabolomics of plant tissues using reverse-phase liquid chromatography connected to high-resolution mass spectrometry (LC-MS) of dilute methanolic extract. MZmine is a specialized computer software that automates the alignment and baseline modification of all derived mass peaks across all samples, resulting in precise information on the relative abundance of hundreds of metabolites reflected by thousands of mass signals. Further processing with statistic and bioinformatic techniques will provide a comprehensive perspective of the variations and connections among groups of samples.
摘要:
胁迫可以影响不同群体的植物代谢产物和多个信号通路。非靶向代谢组学使得能够收集在该时间点存在于植物组织中的全部代谢物含量的全光谱数据。我们为大规模,植物组织的非靶向代谢组学使用反相液相色谱连接到稀甲醇提取物的高分辨率质谱(LC-MS)。MZmine是一款专门的计算机软件,可自动对所有样品中的所有衍生质量峰进行对齐和基线修改,从而产生关于数千种质量信号所反映的数百种代谢物相对丰度的精确信息。使用统计和生物信息学技术进行进一步处理将为样本组之间的变化和联系提供全面的视角。