PDF(Pubmed)
Abstract：
This study aimed to synthesize, characterize, and evaluate the effect of cocamidopropyl betaine-stabilized MnO2 nanoparticles (NPs) on the germination and development of pea seedlings. The synthesized NPs manifested as aggregates ranging from 50-600 nm, comprising spherical particles sized between 19 to 50 nm. These particles exhibited partial crystallization, indicated by peaks at 2θ = 25.37, 37.62, 41.18, 49.41, 61.45, and 65.79°, characteristic of MnO2 with a tetragonal crystal lattice with a I4/m spatial group. Quantum chemical modelling showed that the stabilization process of MnO2 NPs with cocamidopropyl betaine is energetically advantageous (∆E > 1299.000 kcal/mol) and chemically stable, as confirmed by the positive chemical hardness values (0.023 ≤ η ≤ 0.053 eV). It was revealed that the interaction between the MnO2 molecule and cocamidopropyl betaine, facilitated by a secondary amino group (NH), is the most probable scenario. This ascertain is supported by the values of the difference in total energy (∆E = 1299.519 kcal/mol) and chemical hardness (η = 0.053 eV). These findings were further confirmed using FTIR spectroscopy. The effect of MnO2 NPs at various concentrations on the germination of pea seeds was found to be nonlinear and ambiguous. The investigation revealed that MnO2 NPs at a concentration of 0.1 mg/L resulted in the highest germination energy (91.25%), germinability (95.60%), and lengths of roots and seedlings among all experimental samples. However, an increase in the concentration of preparation led to a slight growth suppression (1-10 mg/L) and the pronounced inhibition of seedling and root development (100 mg/L). The analysis of antioxidant indicators and phytochemicals in pea seedlings indicated that only 100 mg/L MnO2 NPs have a negative effect on the content of soluble sugars, chlorophyll a/b, carotenoids, and phenols. Conversely, lower concentrations showed a stimulating effect on photosynthesis indicators. Nevertheless, MnO2 NPs at all concentrations generally decreased the antioxidant potential of pea seedlings, except for the ABTS parameter. Pea seedlings showed a notable capacity to absorb Mn, reaching levels of 586.5 μg/L at 10 mg/L and 892.6 μg/L at 100 mg/L MnO2 NPs, surpassing the toxic level for peas according to scientific literature. However, the most important result was the observed growth-stimulating activity at 0.1 mg/L MnO2 NPs stabilized with cocamidopropyl betaine, suggesting a promising avenue for further research.
摘要：
本研究旨在综合，表征，并评估了椰油酰胺丙基甜菜碱稳定的MnO2纳米颗粒（NPs）对豌豆幼苗萌发和发育的影响。合成的NP表现为50-600nm的聚集体，包括尺寸在19至50nm之间的球形颗粒。这些颗粒表现出部分结晶，由2θ=25.37、37.62、41.18、49.41、61.45和65.79°处的峰指示，MnO2的特征为具有I4/m空间群的四方晶格。量子化学模型表明，MnO2NPs与椰油酰胺丙基甜菜碱的稳定过程在能量上是有利的（ΔE>1299.000kcal/mol），并且在化学上是稳定的，正的化学硬度值(0.023≤η≤0.053eV)证实了这一点。揭示了MnO2分子与椰油酰胺丙基甜菜碱之间的相互作用，由仲氨基(NH)促进，是最可能的情况.这种确定得到了总能量（ΔE=1299.519kcal/mol）和化学硬度（η=0.053eV）的差值的支持。使用FTIR光谱进一步证实了这些发现。发现各种浓度的MnO2NPs对豌豆种子发芽的影响是非线性且模糊的。调查显示，浓度为0.1mg/L的MnO2NPs导致最高的发芽能量（91.25％），发芽性（95.60%），以及所有实验样品中根和幼苗的长度。然而,制剂浓度的增加导致轻微的生长抑制（1-10mg/L），并显着抑制幼苗和根系发育（100mg/L）。对豌豆幼苗的抗氧化指标和植物化学物质的分析表明，只有100mg/L的MnO2NPs对可溶性糖含量有负面影响，叶绿素a/b,类胡萝卜素，和酚类。相反，低浓度对光合作用指标有刺激作用。然而，所有浓度的MnO2NPs通常会降低豌豆幼苗的抗氧化潜力，ABTS参数除外。豌豆幼苗显示出显着的吸收锰的能力，在10mg/L时达到586.5μg/L，在100mg/L时达到892.6μg/L的MnO2NPs，根据科学文献，超过了豌豆的毒性水平。然而,最重要的结果是在用椰油酰胺丙基甜菜碱稳定的0.1mg/LMnO2NPs时观察到的生长刺激活性，为进一步研究提供了一个有希望的途径。
