METHODS: So we used metagenomic next generation sequencing (mNGS) to explore the microbiome differences between the two groups.
RESULTS: Thirty-six CAP without COPD and 11 CAP with COPD cases were retrieved. Bronchoalveolar lavage fluid (BALF) was collected and analyzed using untargeted mNGS and bioinformatic analysis. mNGS revealed that CAP with COPD group was abundant with Streptococcus, Prevotella, Bordetella at genus level and Cutibacterium acnes, Rothia mucilaginosa, Bordetella genomosp. 6 at species level. While CAP without COPD group was abundant with Ralstonia, Prevotella, Streptococcus at genus level and Ralstonia pickettii, Rothia mucilaginosa, Prevotella melaninogenica at species level. Meanwhile, both alpha and beta microbiome diversity was similar between groups. Linear discriminant analysis found that pa-raburkholderia, corynebacterium tuberculostearicum and staphylococcus hominis were more enriched in CAP without COPD group while the abundance of streptococcus intermedius, streptococcus constellatus, streptococcus milleri, fusarium was higher in CAP with COPD group.
CONCLUSIONS: These findings revealed that concomitant COPD have an mild impact on lower airway microbiome of CAP patients.
方法:因此,我们使用宏基因组下一代测序(mNGS)来探索两组之间的微生物组差异。
结果:共检索到36例无COPDCAP和11例COPDCAP病例。收集支气管肺泡灌洗液(BALF)并使用非靶向mNGS和生物信息学分析进行分析。mNGS显示CAP合并COPD组富含链球菌,普雷沃氏菌,属水平的博德特氏菌和痤疮杆菌,粘胶红花,基因博德特氏菌。6在物种水平。虽然无COPD的CAP组有丰富的Ralstonia,普雷沃氏菌,属水平的链球菌和皮克蒂拉尔斯托,粘胶红花,物种水平的黑色素prevotella。同时,两组之间的α和β微生物组多样性相似.线性判别分析发现,pa-raburkholderia,在无COPD的CAP组中,结核杆菌和人葡萄球菌的含量更高,而中间链球菌的含量更高,星座链球菌,milleri链球菌,CAP合并COPD组镰刀菌较高。
结论:这些研究结果表明,合并COPD对CAP患者的下气道微生物组有轻微影响。