Abstract：
The traditional recognition of extracellular matrix (ECM) at tissue sections relies on the time-consuming immunofluorescence that could not meet the demand of rapid diagnosis. Herein, we introduce a thickness-resolved electrochemiluminescence (ECL) microscopy to image thin-layer ECM at tissue sections for fast histopathological analysis. The unique surface-confined ECL mechanism enables to unveil the diversity and complexity of multiple tissue structures with varying thicknesses. Notably, the short lifetimes and the limited diffusion of electrogenerated coreactant radicals combined with their chemical reactivity result in a 2-fold increase in ECL intensity on ECM structures compared to the remaining tissue, enabling ECM visualization without specific labeling. The further quantitation of the ECM localization within tissue sections furnishes crucial insights into tumor progression and, more importantly, differentiates carcinoma and paracancerous tissues from patients in less than 30 min. Moreover, the reported electrochemistry-based microscopy is a dynamic approach allowing to investigate the transport, tortuosity, and trafficking properties through the tissues. This thickness-resolved recognition strategy not only opens new avenues for imaging complex samples but also holds promise for expediting tissue pathologic diagnosis, offering a more automated protocol with enhanced quantitative data compared to current intraoperative pathology methods.
摘要：
传统的在组织切片中识别细胞外基质（ECM）依赖于耗时的免疫荧光，无法满足快速诊断的需求。在这里，我们采用厚度分辨电化学发光(ECL)显微镜对组织切片的薄层ECM进行成像,以进行快速组织病理学分析.独特的表面约束ECL机制能够揭示具有不同厚度的多个组织结构的多样性和复杂性。值得注意的是,短的寿命和有限的扩散电生成的共反应物自由基结合他们的化学反应性导致2倍的ECL强度增加ECM结构相比，其余的组织。启用ECM可视化，无需特定标签。组织切片中ECM定位的进一步定量提供了对肿瘤进展的重要见解，更重要的是,在不到30分钟内将癌和癌旁组织与患者区分开。此外，报道的基于电化学的显微镜是一种动态的方法，允许研究运输，弯曲，并通过组织贩运财产。这种厚度分辨识别策略不仅为复杂样本成像开辟了新途径，而且有望加快组织病理诊断。与目前的术中病理学方法相比，提供了更自动化的方案，并增强了定量数据。
