Abstract：
A Gram-stain-positive, strictly anaerobic, endospore-forming and rod-shaped (0.6-0.8×2.7-13.1 µm) bacterium, designated as 5 N-1T, was isolated from a yellow water sample collected from the manufacturing process of Nongxiangxing baijiu in the Yibin region of Sichuan, PR China. Growth occurred at 15-40 °C (optimum growth at 37 °C), at pH 6.0-9.0 (optimum growth at pH 7.0) and in NaCl concentrations of 0-1 % (w/v) and ethanol concentrations of 0-2 % (v/v). The major fatty acids in strain 5 N-1T were C16 : 0, C18 : 0 and C14 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified aminophospholipids and one unidentified lipid. Phylogenetic analysis of its 16S rRNA gene sequence indicated that strain 5 N-1T was most closely related to Clostridium weizhouense YB-6T (97.70 %) and Clostridium uliginosum DSM 12992T (97.56 %). The average nucleotide identity and digital DNA‒DNA hybridization values between strain 5 N-1T and the above two type strains were 80.89 and 80.05 % and 25.80 and 25.30 %, respectively, which were all below the species thresholds. The genome size of strain 5 N-1T was 3.5 Mbp and the DNA G+C content was 27.5 mol%. Based on the results of phenotypic and genotypic analyses, strain 5 N-1T represents a novel species of the genus Clostridium, for which the name Clostridium aquiflavi sp. nov. is proposed. The type strain is Clostridium aquiflavi 5 N-1T (=CICC 24886T=JCM 35355T).
摘要：
革兰氏染色阳性，严格厌氧，内生孢子形成和杆状（0.6-0.8×2.7-13.1µm）细菌，指定为5N-1T，从四川宜宾地区农香星白酒生产过程中采集的黄水样品中分离出来，公关中国。生长发生在15-40°C（最佳生长在37°C），在pH6.0-9.0（pH7.0的最佳生长）和NaCl浓度为0-1％（w/v）和乙醇浓度为0-2％（v/v）时。菌株5N-1T的主要脂肪酸为C16:0、C18:0和C14:0。极性脂质是二磷脂酰甘油，磷脂酰甘油，磷脂酰乙醇胺，四种身份不明的氨基磷脂和一种身份不明的脂质。对其16SrRNA基因序列的系统发育分析表明，菌株5N-1T与魏氏梭菌YB-6T（97.70％）和梭菌DSM12992T（97.56％）最密切相关。菌株5N-1T与上述两种类型菌株的平均核苷酸同一性和数字DNA-DNA杂交值分别为80.89和80.05%和25.80和25.30%,分别,都低于物种阈值。菌株5N-1T的基因组大小为3.5Mbp，DNAGC含量为27.5mol%。根据表型和基因型分析的结果，菌株5N-1T代表梭菌属的一种新物种，其名称为aquatflavi梭菌。11月。是提议的。菌株类型为水黄梭菌5N-1T(=CICC24886T=JCM35355T)。
