UNASSIGNED: The DBA/1J mice were utilized to establish a collagen-induced RA (CIA) mouse model and to validate the therapeutic efficacy of hUC-MSCs transfected with CD151 siRNA. RNA-seq, QT-PCR and western blotting were utilized to evaluate the mRNA and protein levels of the PI3K/AKT pathway, respectively.
UNASSIGNED: IFN-γ significantly enhanced the proliferation and migration abilities of hUC-MSCs, up-regulating the expression of CD151, a gene related to cell proliferation and migration. Effective inhibition of this effect was achieved through CD151 siRNA treatment. However, IFN-γ did not affect hUC-MSCs differentiation or changes in cell surface markers. Additionally, transplantation of CD151-interfered hUC-MSCs (siRNA-CD151-hUC-MSCs) resulted in decreased colonization in the toes of CIA mice and worse therapeutic effects compared to empty vector treatment (siRNA-NC-hUC-MSCs).
UNASSIGNED: IFN-γ facilitates the proliferation and migration of hUC-MSCs through the CD151/PI3K/AKT pathway. The therapeutic efficacy of siRNA-CD151-hUC-MSCs was found to be inferior to that of siRNA-NC-hUC-MSCs.
■利用DBA/1J小鼠建立胶原诱导的RA(CIA)小鼠模型并验证用CD151siRNA转染的hUC-MSC的治疗功效。RNA-seq,QT-PCR和蛋白质印迹用于评估PI3K/AKT途径的mRNA和蛋白质水平。分别。
■IFN-γ显著增强hUC-MSCs的增殖和迁移能力,上调与细胞增殖和迁移相关的基因CD151的表达。通过CD151siRNA处理实现了这种作用的有效抑制。然而,IFN-γ不影响hUC-MSCs分化或细胞表面标志物的变化。此外,与空载体治疗(siRNA-NC-hUC-MSCs)相比,CD151干扰的hUC-MSCs(siRNA-CD151-hUC-MSCs)移植导致CIA小鼠脚趾定植减少,治疗效果更差.
■IFN-γ通过CD151/PI3K/AKT途径促进hUC-MSCs的增殖和迁移。发现siRNA-CD151-hUC-MSC的治疗功效劣于siRNA-NC-hUC-MSC的治疗功效。