{Reference Type}: Journal Article
{Title}: The proliferation/migration ability mediated by CD151/PI3K/AKT pathway determines the therapeutic effect of hUC-MSCs transplantation on rheumatoid arthritis.
{Author}: Xia X;Shen P;Yang G;Yao M;Wu X;Lyu L;He Y;Li Z;Wang W;Yang Y;Ao X;Xia C;Chen Z;Xu X;
{Journal}: Clin Exp Hypertens
{Volume}: 46
{Issue}: 1
{Year}: 2024 Dec 31
{Factor}: 2.088
{DOI}: 10.1080/10641963.2024.2366270
{Abstract}: <B>UNASSIGNED: </B>To elucidate the underlying mechanism by which the proliferation and migration abilities of human umbilical cord mesenchymal stem cells (hUC-MSCs) determine their therapeutic efficacy in rheumatoid arthritis treatment.<BR><B>UNASSIGNED: </B>The DBA/1J mice were utilized to establish a collagen-induced RA (CIA) mouse model and to validate the therapeutic efficacy of hUC-MSCs transfected with CD151 siRNA. RNA-seq, QT-PCR and western blotting were utilized to evaluate the mRNA and protein levels of the PI3K/AKT pathway, respectively.<BR><B>UNASSIGNED: </B>IFN-γ significantly enhanced the proliferation and migration abilities of hUC-MSCs, up-regulating the expression of CD151, a gene related to cell proliferation and migration. Effective inhibition of this effect was achieved through CD151 siRNA treatment. However, IFN-γ did not affect hUC-MSCs differentiation or changes in cell surface markers. Additionally, transplantation of CD151-interfered hUC-MSCs (siRNA-CD151-hUC-MSCs) resulted in decreased colonization in the toes of CIA mice and worse therapeutic effects compared to empty vector treatment (siRNA-NC-hUC-MSCs).<BR><B>UNASSIGNED: </B>IFN-γ facilitates the proliferation and migration of hUC-MSCs through the CD151/PI3K/AKT pathway. The therapeutic efficacy of siRNA-CD151-hUC-MSCs was found to be inferior to that of siRNA-NC-hUC-MSCs.