PDF(Pubmed)
Abstract：
BACKGROUND: Hepatic ischemia/reperfusion (I/R) injury is a significant clinical condition that can arise during liver resections, trauma, and shock. Geraniol, an isoterpene molecule commonly found in nature, possesses antioxidant and hepatoprotective properties. This study investigates the impact of geraniol on hepatic damage by inducing experimental liver I/R injury in rats.
METHODS: Twenty-eight male Wistar Albino rats weighing 350-400 g were utilized for this study. The rats were divided into four groups: control group, I/R group, 50 mg/kg geraniol+I/R group, and 100 mg/kg geraniol+I/R group. Ischemia times were set at 15 minutes with reperfusion times at 20 minutes. Ischemia commenced 15 minutes after geraniol administration. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactic acid were measured, along with superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity levels in liver tissues. Liver tissues were also examined histopathologically.
RESULTS: It was observed that intraperitoneal administration of 50 mg/kg and 100 mg/kg geraniol significantly reduced AST, lactic acid, and tumor necrosis factor-alpha (TNF-α) levels. The serum ALT level decreased significantly in the 50 mg/kg group, whereas no significant decrease was found in the 100 mg/kg group. SOD and GPx enzyme activities were shown to increase significantly in the 100 mg/kg group. Although there was an increase in these enzyme levels in the 50 mg/kg group, it was not statistically significant. Similarly, CAT enzyme activity increased in both the 50 mg/kg and 100 mg/kg groups, but the increase was not significant. The Suzuki score significantly decreased in both the 50 mg/kg and 100 mg/kg groups.
CONCLUSIONS: The study demonstrates that geraniol reduced hepatic damage both biochemically and histopathologically and increased antioxidant defense enzymes. These findings suggest that geraniol could be used to prevent hepatic I/R injury, provided it is corroborated by large-scale and comprehensive studies.
METHODS: Twenty-eight male Wistar Albino rats weighing 350-400 g were utilized for this study. The rats were divided into four groups: control group, I/R group, 50 mg/kg geraniol+I/R group, and 100 mg/kg geraniol+I/R group. Ischemia times were set at 15 minutes with reperfusion times at 20 minutes. Ischemia commenced 15 minutes after geraniol administration. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactic acid were measured, along with superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity levels in liver tissues. Liver tissues were also examined histopathologically.
RESULTS: It was observed that intraperitoneal administration of 50 mg/kg and 100 mg/kg geraniol significantly reduced AST, lactic acid, and tumor necrosis factor-alpha (TNF-α) levels. The serum ALT level decreased significantly in the 50 mg/kg group, whereas no significant decrease was found in the 100 mg/kg group. SOD and GPx enzyme activities were shown to increase significantly in the 100 mg/kg group. Although there was an increase in these enzyme levels in the 50 mg/kg group, it was not statistically significant. Similarly, CAT enzyme activity increased in both the 50 mg/kg and 100 mg/kg groups, but the increase was not significant. The Suzuki score significantly decreased in both the 50 mg/kg and 100 mg/kg groups.
CONCLUSIONS: The study demonstrates that geraniol reduced hepatic damage both biochemically and histopathologically and increased antioxidant defense enzymes. These findings suggest that geraniol could be used to prevent hepatic I/R injury, provided it is corroborated by large-scale and comprehensive studies.
摘要：
背景：肝缺血/再灌注（I/R）损伤是在肝切除过程中可能出现的重要临床状况，创伤,和震惊。香叶醇,是自然界中常见的一种同萜分子,具有抗氧化和保肝性能。本研究通过诱导大鼠实验性肝I/R损伤来研究香叶醇对肝损伤的影响。
方法:本研究使用重350-400g的28只雄性Wistar白化大鼠。将大鼠分为4组:对照组,I/R组,50mg/kg香叶醇+I/R组，和100mg/kg香叶醇+I/R组。缺血时间设定为15分钟,再灌注时间设定为20分钟。在施用香叶醇后15分钟开始缺血。血清丙氨酸转氨酶（ALT）水平，天冬氨酸转氨酶（AST），和乳酸被测量，随着超氧化物歧化酶（SOD），过氧化氢酶(CAT),和肝脏组织中的谷胱甘肽过氧化物酶（GPx）活性水平。还对肝组织进行了组织病理学检查。
结果：观察到腹膜内施用50mg/kg和100mg/kg香叶醇显着降低AST，乳酸,和肿瘤坏死因子-α（TNF-α）水平。50mg/kg组血清ALT水平明显下降，而在100mg/kg组中没有发现显着下降。在100mg/kg组中，SOD和GPx酶活性显着增加。尽管在50mg/kg组中这些酶水平有所增加，没有统计学意义。同样，50mg/kg和100mg/kg组的CAT酶活性均增加，但增幅不大。50mg/kg和100mg/kg组的Suzuki评分均显着降低。
结论：研究表明，香叶醇在生化和组织病理学上都能减少肝损伤，并增加抗氧化防御酶。这些结果表明，香叶醇可用于预防肝I/R损伤，只要得到大规模和全面研究的证实。
方法:本研究使用重350-400g的28只雄性Wistar白化大鼠。将大鼠分为4组:对照组,I/R组,50mg/kg香叶醇+I/R组，和100mg/kg香叶醇+I/R组。缺血时间设定为15分钟,再灌注时间设定为20分钟。在施用香叶醇后15分钟开始缺血。血清丙氨酸转氨酶（ALT）水平，天冬氨酸转氨酶（AST），和乳酸被测量，随着超氧化物歧化酶（SOD），过氧化氢酶(CAT),和肝脏组织中的谷胱甘肽过氧化物酶（GPx）活性水平。还对肝组织进行了组织病理学检查。
结果：观察到腹膜内施用50mg/kg和100mg/kg香叶醇显着降低AST，乳酸,和肿瘤坏死因子-α（TNF-α）水平。50mg/kg组血清ALT水平明显下降，而在100mg/kg组中没有发现显着下降。在100mg/kg组中，SOD和GPx酶活性显着增加。尽管在50mg/kg组中这些酶水平有所增加，没有统计学意义。同样，50mg/kg和100mg/kg组的CAT酶活性均增加，但增幅不大。50mg/kg和100mg/kg组的Suzuki评分均显着降低。
结论：研究表明，香叶醇在生化和组织病理学上都能减少肝损伤，并增加抗氧化防御酶。这些结果表明，香叶醇可用于预防肝I/R损伤，只要得到大规模和全面研究的证实。
