Abstract：
Polycystic ovary syndrome (PCOS) is a multidisciplinary endocrinopathy that affects women of reproductive age. It is characterized by menstrual complications, hyperandrogenism, insulin resistance, and cardiovascular issues. The current research investigated the efficacy of rosmarinic acid in letrozole-induced PCOS in adult female rats as well as the potential underlying molecular mechanisms. Forty female rats were divided into the control group, the rosmarinic acid group (50 mg/kg per orally, po) for 21 days, PCOS group; PCOS was induced by administration of letrozole (1 mg/kg po) for 21 days, and rosmarinic acid-PCOS group, received rosmarinic acid after PCOS induction. PCOS resulted in a marked elevation in both serum luteinizing hormone (LH) and testosterone levels and LH/follicle-stimulating hormone ratio with a marked reduction in serum estradiol and progesterone levels. A marked rise in tumor necrosis factor-α (TNF-α), interleukin-1β, monocyte chemotactic protein-1, and vascular endothelial growth factor (messenger RNA) in the ovarian tissue was reported. The histological analysis displayed multiple cystic follicles in the ovarian cortex with markedly thin granulosa cell layer, vacuolated granulosa and theca cell layers, and desquamated granulosa cells. Upregulation in the immune expression of TNF-α and caspase-3 was demonstrated in the ovarian cortex. Interestingly, rosmarinic acid ameliorated the biochemical and histopathological changes. In conclusion, rosmarinic acid ameliorates letrozole-induced PCOS through its anti-inflammatory and antiangiogenesis effects.
摘要：
多囊卵巢综合征（PCOS）是一种多学科内分泌疾病，影响育龄妇女。它的特点是月经并发症，雄激素过多症,胰岛素抵抗，和心血管问题。本研究探讨了迷迭香酸在成年雌性大鼠来曲唑诱导的PCOS中的疗效以及潜在的分子机制。将40只雌性大鼠分为对照组,迷迭香酸组(每次口服50mg/kg，po)21天，PCOS组；给予来曲唑（1mg/kgpo）21天诱导PCOS，迷迭香酸-PCOS组，PCOS诱导后接受迷迭香酸。PCOS导致血清黄体生成素（LH）和睾丸激素水平以及LH/卵泡刺激素比例显着升高，血清雌二醇和孕酮水平显着降低。肿瘤坏死因子-α(TNF-α)显著升高,白细胞介素-1β，报道了卵巢组织中的单核细胞趋化蛋白-1和血管内皮生长因子（信使RNA）。组织学分析显示卵巢皮质中有多个囊性卵泡，颗粒细胞层明显薄，空泡颗粒细胞和卵泡膜细胞层，颗粒细胞脱落.在卵巢皮质中证明了TNF-α和caspase-3的免疫表达上调。有趣的是,迷迭香酸改善了生化和组织病理学变化。总之,迷迭香酸通过抗炎和抗血管生成作用改善来曲唑诱导的PCOS。
