PDF(Pubmed)
Abstract：
A detailed understanding of how spaceflight affects human health is essential for long-term space exploration. Liquid biopsies allow for minimally-invasive multi-omics assessments that can resolve the molecular heterogeneity of internal tissues. Here, we report initial results from the JAXA Cell-Free Epigenome Study, a liquid biopsy study with six astronauts who resided on the International Space Station (ISS) for more than 120 days. Analysis of plasma cell-free RNA (cfRNA) collected before, during, and after spaceflight confirms previously reported mitochondrial dysregulation in space. Screening with 361 cell surface marker antibodies identifies a mitochondrial DNA-enriched fraction associated with the scavenger receptor CD36. RNA-sequencing of the CD36 fraction reveals tissue-enriched RNA species, suggesting the plasma mitochondrial components originated from various tissues. We compare our plasma cfRNA data to mouse plasma cfRNA data from a previous JAXA mission, which had used on-board artificial gravity, and discover a link between microgravity and the observed mitochondrial responses.
摘要：
详细了解太空飞行如何影响人类健康对于长期太空探索至关重要。液体活检允许微创多组学评估，可以解决内部组织的分子异质性。这里,我们报告了JAXA无细胞表观基因组研究的初步结果，对六名在国际空间站（ISS）居住超过120天的宇航员进行的液体活检研究。分析之前收集的血浆无细胞RNA（cfRNA），during,太空飞行后证实了先前报道的太空线粒体失调。用361个细胞表面标记物抗体筛选鉴定了与清道夫受体CD36相关的线粒体DNA富集级分。CD36部分的RNA测序揭示了组织富集的RNA种类，表明血浆线粒体成分起源于各种组织。我们将我们的血浆cfRNA数据与之前JAXA任务的小鼠血浆cfRNA数据进行了比较，使用了车载人工重力，并发现微重力和观察到的线粒体反应之间的联系。
