PDF(Pubmed)
Abstract：
BACKGROUND: Contamination in the contact lens training area could be due to bacteria, which can lead to the major consequence of ocular infections. We aimed to investigate the contamination caused by bacteria in the contact lens training area in private optical clinics of the Udupi district, India.
METHODS: A cross-sectional study evaluated the swabs from the contact lens container, contact lens solution tip, washing area and lens fitting area for bacterial contamination. Twenty swabs collected from different areas of five optical clinics were inoculated in Brain heart infusion broth (BHIB). The broth was streaked in MacConkey and Blood agar and incubated at standard conditions for the growth of bacteria. All isolates were identified using conventional culture methods, and Gram staining was performed.
RESULTS: Twenty samples (contact lens case, n = 5; contact lens solution tip, n = 5; washing area, n = 5; cleaning towel, n = 5) from private optical clinics were recruited for the study. Bacterial growth was indicated in which lactose fermentation was seen at (15%), non-lactose fermentation at (35%), and no bacterial growth at (50%) in MacConkey agar. Partial or alpha-hemolytic (α hemolysis) was seen in (5%), complete or beta-hemolytic (β hemolysis) was seen in (40%), no hemolysis or gamma hemolysis (ϫ haemolysis), was seen in (30%), no growth was seen in (25%) on blood agar. Gram-positive cocci (45%), Gram-negative bacilli (20%), and no increase in (35%) were observed in MacConkey agar and Blood agar. Bacterial species were not identified in this study.
CONCLUSIONS: Contamination was found in lenses, solution tips, washing areas, and cleaning towels which might lead to ocular infections. Perception should be given to those responsible for fitting lenses.
METHODS: A cross-sectional study evaluated the swabs from the contact lens container, contact lens solution tip, washing area and lens fitting area for bacterial contamination. Twenty swabs collected from different areas of five optical clinics were inoculated in Brain heart infusion broth (BHIB). The broth was streaked in MacConkey and Blood agar and incubated at standard conditions for the growth of bacteria. All isolates were identified using conventional culture methods, and Gram staining was performed.
RESULTS: Twenty samples (contact lens case, n = 5; contact lens solution tip, n = 5; washing area, n = 5; cleaning towel, n = 5) from private optical clinics were recruited for the study. Bacterial growth was indicated in which lactose fermentation was seen at (15%), non-lactose fermentation at (35%), and no bacterial growth at (50%) in MacConkey agar. Partial or alpha-hemolytic (α hemolysis) was seen in (5%), complete or beta-hemolytic (β hemolysis) was seen in (40%), no hemolysis or gamma hemolysis (ϫ haemolysis), was seen in (30%), no growth was seen in (25%) on blood agar. Gram-positive cocci (45%), Gram-negative bacilli (20%), and no increase in (35%) were observed in MacConkey agar and Blood agar. Bacterial species were not identified in this study.
CONCLUSIONS: Contamination was found in lenses, solution tips, washing areas, and cleaning towels which might lead to ocular infections. Perception should be given to those responsible for fitting lenses.
摘要：
背景：隐形眼镜训练区的污染可能是由于细菌，这可能导致眼部感染的主要后果。我们旨在调查Udupi区私人光学诊所隐形眼镜训练区细菌造成的污染，印度。
方法：一项横断面研究评估了隐形眼镜容器中的拭子，隐形眼镜溶液尖端，清洗区和镜头装配区的细菌污染。将从五个光学诊所的不同区域收集的20个拭子接种在脑心输注肉汤(BHIB)中。将肉汤在MacConkey和血琼脂中划线,并在用于细菌生长的标准条件下孵育。使用常规培养方法鉴定所有分离株，并进行革兰氏染色。
结果：20个样本（隐形眼镜盒，n=5；隐形眼镜溶液尖端，n=5；冲洗面积，n=5；清洁毛巾，从私人光学诊所招募n=5）进行研究。表明细菌生长，其中乳糖发酵在（15％），非乳糖发酵（35%），并且在MacConkey琼脂中(50%)没有细菌生长。部分或α-溶血（α溶血）见于（5%），完全或β-溶血（β溶血）见于（40%），无溶血或γ溶血（溶血），在(30%)，在(25%)血琼脂上没有看到生长。革兰氏阳性球菌（45%），革兰氏阴性杆菌（20%），在MacConkey琼脂和血液琼脂中没有观察到(35%)增加。在这项研究中没有鉴定出细菌物种。
结论：在镜片中发现污染，解决方案提示,洗涤区,和可能导致眼部感染的清洁毛巾。应该给那些负责验配镜片的人以感知。
方法：一项横断面研究评估了隐形眼镜容器中的拭子，隐形眼镜溶液尖端，清洗区和镜头装配区的细菌污染。将从五个光学诊所的不同区域收集的20个拭子接种在脑心输注肉汤(BHIB)中。将肉汤在MacConkey和血琼脂中划线,并在用于细菌生长的标准条件下孵育。使用常规培养方法鉴定所有分离株，并进行革兰氏染色。
结果：20个样本（隐形眼镜盒，n=5；隐形眼镜溶液尖端，n=5；冲洗面积，n=5；清洁毛巾，从私人光学诊所招募n=5）进行研究。表明细菌生长，其中乳糖发酵在（15％），非乳糖发酵（35%），并且在MacConkey琼脂中(50%)没有细菌生长。部分或α-溶血（α溶血）见于（5%），完全或β-溶血（β溶血）见于（40%），无溶血或γ溶血（溶血），在(30%)，在(25%)血琼脂上没有看到生长。革兰氏阳性球菌（45%），革兰氏阴性杆菌（20%），在MacConkey琼脂和血液琼脂中没有观察到(35%)增加。在这项研究中没有鉴定出细菌物种。
结论：在镜片中发现污染，解决方案提示,洗涤区,和可能导致眼部感染的清洁毛巾。应该给那些负责验配镜片的人以感知。
