Abstract：
Mild whole-body hyperthermia has been shown to have anti-tumor effects through an immune-modulating mechanism. Before it is widely applied in the clinic, tremendous mechanistic research in animals is necessary to adhere to evidence-based principles. The radio frequency electromagnetic field (RF-EMF) based heating facility could be a good choice for hyperthermia treatment, but the heating characteristics of a facility, including structure design, electromagnetic and thermal dosimetry, and the biologic effects of hyperthermia, need to be well elucidated. Here, we reported the heating characteristic study on a resonant chamber (RC) excited by a 1800 MHz solid source. The EMF in the RC was stirred by 24 static reflectors, which resulted in the standard deviation of electric field intensity being below 3 dB in the EM homogeneity evaluation. For the exposure scenario, six free-moving mice were loaded into separate cases and exposed simultaneously in the RC. The EMF energy absorption and distribution in exposed mice were calculated with the 12-plane-waves method of numerical simulation. Different levels of core body temperature increment in exposed mice were achieved through regulation of the source output power. Overexpression of heat shock proteins (HSPs) was detected in the liver, lung and muscle, but not in the brain of the exposed mice. The levels of representative inflammatory cytokines in the serum, TNF-α and IL-10 increased post RC exposure. Based on the heating characteristic study and validation, the applied RC would be a qualified heating system for mild whole-body hyperthermia effect research in mice.
Mild whole-body hyperthermia has potential anti-tumor effects by modulating the immune system. A radio frequency electromagnetic field (RF-EMF)-based heating facility emerges as a suitable option for hyperthermia treatment. However, a qualified heating facility for scientific research must elucidate its heating characteristics and validate the biological effects associated with hyperthermia. In this study, we report the characteristics of a rodent heating chamber using EMF energy. The special structure of the chamber not only achieved efficient EMF usage but also ensured the homogeneity in EMF spatial distribution, animal EM absorption, and EMF-caused biological effects. Our work may offer insights for designing a low-cost yet reliable heating facility for scientific research.
Mild whole-body hyperthermia has potential anti-tumor effects by modulating the immune system. A radio frequency electromagnetic field (RF-EMF)-based heating facility emerges as a suitable option for hyperthermia treatment. However, a qualified heating facility for scientific research must elucidate its heating characteristics and validate the biological effects associated with hyperthermia. In this study, we report the characteristics of a rodent heating chamber using EMF energy. The special structure of the chamber not only achieved efficient EMF usage but also ensured the homogeneity in EMF spatial distribution, animal EM absorption, and EMF-caused biological effects. Our work may offer insights for designing a low-cost yet reliable heating facility for scientific research.
摘要：
已显示轻度全身热疗通过免疫调节机制具有抗肿瘤作用。在临床广泛应用之前,在动物中进行大量的机械研究对于坚持循证原则是必要的。基于射频电磁场（RF-EMF）的加热设施可能是热疗治疗的好选择，但是设施的供暖特性，包括结构设计，电磁和热剂量测定，以及热疗的生物学效应，需要很好地阐明。这里,我们报道了由1800MHz固体源激发的谐振腔（RC）的加热特性研究。RC中的EMF由24个静态反射器搅拌，这导致在EM均匀性评估中电场强度的标准偏差低于3dB。对于曝光场景，将6只自由移动的小鼠加载到单独的病例中，并同时暴露在RC中。用数值模拟的12平面波方法计算了暴露小鼠的EMF能量吸收和分布。通过调节源输出功率，可以实现暴露小鼠体内不同水平的核心体温升高。在肝脏中检测到热休克蛋白（HSPs）的过表达，肺和肌肉，但不在暴露小鼠的大脑中。血清中代表性炎性细胞因子的水平，TNF-α和IL-10在RC暴露后增加。在加热特性研究和验证的基础上，所应用的RC将是用于小鼠轻度全身热疗效应研究的合格加热系统。
轻度全身热疗通过调节免疫系统具有潜在的抗肿瘤作用。基于射频电磁场（RF-EMF）的加热设施成为热疗的合适选择。然而,用于科学研究的合格加热设施必须阐明其加热特性，并验证与热疗相关的生物学效应。在这项研究中,我们报告了使用EMF能量的啮齿动物加热室的特征。腔室的特殊结构不仅实现了有效的EMF使用，而且确保了EMF空间分布的均匀性，动物EM吸收，和EMF引起的生物效应。我们的工作可能会为为科学研究设计低成本但可靠的加热设施提供见解。
轻度全身热疗通过调节免疫系统具有潜在的抗肿瘤作用。基于射频电磁场（RF-EMF）的加热设施成为热疗的合适选择。然而,用于科学研究的合格加热设施必须阐明其加热特性，并验证与热疗相关的生物学效应。在这项研究中,我们报告了使用EMF能量的啮齿动物加热室的特征。腔室的特殊结构不仅实现了有效的EMF使用，而且确保了EMF空间分布的均匀性，动物EM吸收，和EMF引起的生物效应。我们的工作可能会为为科学研究设计低成本但可靠的加热设施提供见解。
