RESULTS: Here, we present a chromosome-level genomic assembly of rose myrtle (genome size = 466 Mb, scaffold N50 = 43.7 Mb) with 35,554 protein-coding genes predicted. Through comparative genomic analysis, we found that gene expansion and duplication had a potential contribution to the accumulation of volatile substances. We proposed that the action of positive selection was significantly involved in volatile accumulation. We identified 43 TPS genes in R. tomentosa. Further transcriptomic and TPS gene family analyses demonstrated that the distinct gene subgroups of TPS may contribute greatly to the biosynthesis and accumulation of different volatiles in the Myrtle family of shrubs and trees. The results suggested that the diversity of TPS-a subgroups led to the accumulation of special sesquiterpenes in different plants of the Myrtaceae family.
CONCLUSIONS: The high quality chromosome-level rose myrtle genome and the comparative analysis of TPS gene family open new avenues for obtaining a higher commercial value of essential oils in medical plants.
结果:这里,我们提出了玫瑰桃金娘的染色体水平基因组组装(基因组大小=466Mb,支架N50=43.7Mb),预测了35,554个蛋白质编码基因。通过比较基因组分析,我们发现基因扩增和重复对挥发性物质的积累有潜在的贡献。我们提出,正选择的作用显着参与挥发性积累。我们鉴定了毛霉素中的43个TPS基因。进一步的转录组和TPS基因家族分析表明,TPS的不同基因亚组可能对默特尔灌木和树木家族中不同挥发物的生物合成和积累做出了巨大贡献。结果表明,TPS-a亚组的多样性导致了桃金娘科不同植物中特殊倍半萜的积累。
结论:高质量的染色体水平玫瑰桃金娘基因组和TPS基因家族的比较分析为在药用植物中获得更高商业价值的精油开辟了新途径。