Abstract：
BACKGROUND: Regional lymph nodes are frequently sampled in cats with suspected intestinal lymphoma; however, their diagnostic value has not been explored.
OBJECTIVE: To investigate whether histologic and immunohistochemical analysis of mesenteric lymph nodes correlates with the diagnosis of intestinal lymphoma in cats.
METHODS: One hundred 2 client-owned cats diagnosed with intestinal lymphoma.
METHODS: Retrospective study. The inclusion criteria required a full-thickness biopsy of the small intestine and concurrent excision of mesenteric lymph nodes. Histologic and immunophenotypic analyses were performed on intestinal biopsies and corresponding lymph nodes. Selected nodal samples diagnosed with reactive lymph nodes underwent clonality testing.
RESULTS: Transmural T-cell lymphomas, encompassing small and large cell types, were predominant (64 cases, 62.7%), with large B-cell lymphomas being more frequently transmural (68.8%) than mucosal (31.2%). Among all lymph nodes examined, 44 (43.1%; 95% CI: 33.9%-52.8%) exhibited neoplastic infiltration. Among cases of small cell lymphoma, 51 out of 72 (70.8%; 95% CI: 59.4%-80.1%) showed no nodal involvement. Clonality results correctly identified 19/30 (63.3%; 95% CI: 45.5%-78.2%) reactive lymph nodes. Concerns were raised regarding clonal identification in the remaining cases and potential misdiagnoses based on phenotypic characteristics.
CONCLUSIONS: The study underscores the potential drawbacks of relying solely on mesenteric lymph nodes for diagnosing intestinal lymphomas in cats, particularly small cell subtypes. It emphasizes the importance of full-thickness biopsies for assessing transmural infiltration and recommends caution when utilizing mesenteric lymph nodes for histologic, immunohistochemical and clonality evaluations in mucosal lymphomas. Despite limitations, this research highlights the need for comprehensive diagnostic strategies in cats with intestinal lymphoma.
OBJECTIVE: To investigate whether histologic and immunohistochemical analysis of mesenteric lymph nodes correlates with the diagnosis of intestinal lymphoma in cats.
METHODS: One hundred 2 client-owned cats diagnosed with intestinal lymphoma.
METHODS: Retrospective study. The inclusion criteria required a full-thickness biopsy of the small intestine and concurrent excision of mesenteric lymph nodes. Histologic and immunophenotypic analyses were performed on intestinal biopsies and corresponding lymph nodes. Selected nodal samples diagnosed with reactive lymph nodes underwent clonality testing.
RESULTS: Transmural T-cell lymphomas, encompassing small and large cell types, were predominant (64 cases, 62.7%), with large B-cell lymphomas being more frequently transmural (68.8%) than mucosal (31.2%). Among all lymph nodes examined, 44 (43.1%; 95% CI: 33.9%-52.8%) exhibited neoplastic infiltration. Among cases of small cell lymphoma, 51 out of 72 (70.8%; 95% CI: 59.4%-80.1%) showed no nodal involvement. Clonality results correctly identified 19/30 (63.3%; 95% CI: 45.5%-78.2%) reactive lymph nodes. Concerns were raised regarding clonal identification in the remaining cases and potential misdiagnoses based on phenotypic characteristics.
CONCLUSIONS: The study underscores the potential drawbacks of relying solely on mesenteric lymph nodes for diagnosing intestinal lymphomas in cats, particularly small cell subtypes. It emphasizes the importance of full-thickness biopsies for assessing transmural infiltration and recommends caution when utilizing mesenteric lymph nodes for histologic, immunohistochemical and clonality evaluations in mucosal lymphomas. Despite limitations, this research highlights the need for comprehensive diagnostic strategies in cats with intestinal lymphoma.
摘要：
背景：在疑似肠淋巴瘤的猫中经常采样区域淋巴结；然而，其诊断价值尚未被探索。
目的：探讨肠系膜淋巴结的组织学和免疫组织化学分析是否与猫肠淋巴瘤的诊断相关。
方法：一百零二只被诊断为肠淋巴瘤的客户拥有的猫。
方法：回顾性研究。纳入标准要求对小肠进行全层活检，并同时切除肠系膜淋巴结。对肠活检和相应的淋巴结进行了组织学和免疫表型分析。对诊断为反应性淋巴结的选定淋巴结样本进行克隆性测试。
结果：透壁性T细胞淋巴瘤，包括小细胞和大细胞类型，以(64例，62.7%），大B细胞淋巴瘤的透壁性（68.8％）高于粘膜（31.2％）。在所有检查的淋巴结中，44例(43.1%;95%CI:33.9%-52.8%)显示肿瘤浸润。在小细胞淋巴瘤病例中，72人中有51人（70.8%；95%CI：59.4%-80.1%）未显示淋巴结受累。克隆性结果正确识别了19/30（63.3％；95％CI：45.5％-78.2％）反应性淋巴结。对其余病例的克隆鉴定和基于表型特征的潜在误诊提出了担忧。
结论：该研究强调了仅依靠肠系膜淋巴结诊断猫肠道淋巴瘤的潜在缺点。特别是小细胞亚型。它强调了全层活检对评估透壁浸润的重要性，并建议在使用肠系膜淋巴结进行组织学检查时谨慎。粘膜淋巴瘤的免疫组织化学和克隆性评估。尽管有局限性,这项研究强调了猫肠道淋巴瘤的综合诊断策略的必要性。
目的：探讨肠系膜淋巴结的组织学和免疫组织化学分析是否与猫肠淋巴瘤的诊断相关。
方法：一百零二只被诊断为肠淋巴瘤的客户拥有的猫。
方法：回顾性研究。纳入标准要求对小肠进行全层活检，并同时切除肠系膜淋巴结。对肠活检和相应的淋巴结进行了组织学和免疫表型分析。对诊断为反应性淋巴结的选定淋巴结样本进行克隆性测试。
结果：透壁性T细胞淋巴瘤，包括小细胞和大细胞类型，以(64例，62.7%），大B细胞淋巴瘤的透壁性（68.8％）高于粘膜（31.2％）。在所有检查的淋巴结中，44例(43.1%;95%CI:33.9%-52.8%)显示肿瘤浸润。在小细胞淋巴瘤病例中，72人中有51人（70.8%；95%CI：59.4%-80.1%）未显示淋巴结受累。克隆性结果正确识别了19/30（63.3％；95％CI：45.5％-78.2％）反应性淋巴结。对其余病例的克隆鉴定和基于表型特征的潜在误诊提出了担忧。
结论：该研究强调了仅依靠肠系膜淋巴结诊断猫肠道淋巴瘤的潜在缺点。特别是小细胞亚型。它强调了全层活检对评估透壁浸润的重要性，并建议在使用肠系膜淋巴结进行组织学检查时谨慎。粘膜淋巴瘤的免疫组织化学和克隆性评估。尽管有局限性,这项研究强调了猫肠道淋巴瘤的综合诊断策略的必要性。
