Protocol for building an in vitro model of M2-like tumor-associated macrophages with lactic acid or conditioned medium from Lewis cells.

用乳酸或来自 Lewis 细胞的条件培养基构建 M2 样肿瘤相关巨噬细胞的体外模型的方案。

• 发表时间: 2024 Jun 21
• 来源期刊: STAR Protoc
• DOI: 10.1016/j.xpro.2024.103120
• PMID: 38857154
• 文章类型: Journal Article
• 作者列表: Fang X,Wu Y,Qin H,Zhao P,Shan M,Wang F,Li Q

关键词: Cancer; Cell culture; Cell isolation; Immunology

Mesh: Culture Media, Conditioned / pharmacology; Tumor-Associated Macrophages / metabolism drug effects; Lactic Acid / metabolism pharmacology; Animals; Mice; Macrophages / drug effects metabolism; Models, Biological

Abstract

The model of M2-like tumor-associated macrophages (TAMs) is an increasingly attractive model for the study of TAMs. However, the detailed process of M2-like TAMs polarization induced by lactic acid or conditioned medium from Lewis cells (LCM) and the identification of M2-like TAMs is not yet available. In this protocol, we present the detailed methods to induce M2-like TAMs polarization and verify its functionality in order to better carry out related research. For complete details on the use and execution of this protocol, please refer to Fang et al.1.

摘要

M2样肿瘤相关巨噬细胞(TAMs)模型是研究TAMs越来越有吸引力的模型。乳酸或Lewis细胞（LCM）条件培养基诱导的M2样TAM极化的详细过程以及M2样TAM的鉴定尚不可用。在这个协议中,为了更好地开展相关研究，我们提出了诱导M2样TAMs极化的详细方法并验证其功能。有关此协议的使用和执行的完整详细信息，PleaserefertoFangetal.1.