Abstract：
The restoration of adequate function and sensation in nerves following an injury is often insufficient. Electrical stimulation (ES) applied during nerve repair can promote axon regeneration, which may enhance the likelihood of successful functional recovery. However, increasing operation time and complexity are associated with limited clinical use of ES. This study aims to better assess whether short-duration ES types (voltage mode vs. current mode) are able to produce enhanced regenerative activity following peripheral nerve repair in rat models. Wistar rats were randomly divided into 3 groups: no ES (control), 30-minute ES with a current pulse, and 30-minute ES with a voltage pulse. All groups underwent sciatic nerve transection and repair using a silicone tube to bridge the 6-mm gap between the stumps. In the 2 groups other than the control, ES was applied after the surgical repair. Outcomes were evaluated using electrophysiology, histology, and serial walking track analysis. Biweekly walking tracks test over 12 weeks revealed that subjects that underwent ES experienced more rapid functional improvement than subjects that underwent repair alone. Electrophysiological analysis of the newly intratubular sciatic nerve at week 12 revealed strong motor function recovery in rats that underwent 30-minute ES. Histologic analysis of the sciatic nerve and its tibial branch at 12 weeks demonstrated robust axon regrowth in all groups. Both types of short-duration ES applied during nerve repair can promote axon regrowth and enhance the chances of successful functional recovery.
摘要：
损伤后神经的适当功能和感觉的恢复通常是不够的。在神经修复过程中施加电刺激（ES）可以促进轴突再生，这可能会提高成功恢复功能的可能性。然而,手术时间和复杂性的增加与ES的临床应用有限有关.本研究旨在更好地评估短期ES类型(电压模式与当前模式）能够在大鼠模型中周围神经修复后产生增强的再生活动。Wistar大鼠随机分为3组:无ES(对照组),带电流脉冲的30分钟ES，和30分钟的ES与电压脉冲。所有组均接受坐骨神经横切术，并使用硅胶管桥接树桩之间的6毫米间隙进行修复。在对照组以外的2组中，手术修复后应用ES。使用电生理学评估结果，组织学,和串行步行轨迹分析。超过12周的双周步行道测试显示，接受ES的受试者比单独接受修复的受试者经历了更快的功能改善。在第12周时对新的小管内坐骨神经进行的电生理分析显示，接受30分钟ES的大鼠的运动功能恢复良好。12周时坐骨神经及其胫骨分支的组织学分析显示，所有组轴突均有强劲的再生。在神经修复期间应用两种类型的短期ES可以促进轴突再生并增加成功的功能恢复的机会。
