PDF(Pubmed)
Abstract：
Mastophorus muris (Gmelin, 1790) is a globally distributed parasitic nematode of broad range mammals. The taxonomy within the genus Mastophorus and the cryptic diversity among the genus are controversial among taxonomists. This study provides a detailed morphological description of M. muris from Mus musculus combined with a molecular phylogenetic approach. Moreover, descriptions and molecular data of M. muris from non-Mus rodents and wildcats complement our findings and together provide new insights into their taxonomy. The analysis of M. muris was based on light microscopy and scanning electron microscopy. The morphological description focused on the dentition pattern of the two trilobed pseudolabia. Additionally, we described the position of the vulva, arrangement of caudal pairs of papillae, spicules and measured specimens from both sexes and the eggs. For the molecular phylogenetic approach, we amplified the small subunit ribosomal RNA gene and the internal transcribed spacer, and the cytochrome c oxidase subunit 1. Mastophorus morphotypes based on dentition patterns and phylogenetic clustering indicate a subdivision of the genus in agreement with their host. We recognize two groups without a change to formal taxonomy: One group including those specimens infecting Mus musculus, and the second group including organisms infecting non-Mus rodents. Our genetic and morphological data shed light into the cryptic diversity within the genus Mastopohorus. We identified two host-associated groups of M. muris. The described morphotypes and genotypes of M. muris allow a consistent distinction between host-associated parasites.
摘要：
Mastophorusmuris（Gmelin，1790)是一种全球分布广泛的哺乳动物寄生线虫。Mastophorus属的分类学和该属之间的隐秘多样性在分类学家之间存在争议。这项研究结合分子系统发育方法提供了来自小家鼠的M.muris的详细形态学描述。此外，来自非Mus啮齿动物和野猫的M.muris的描述和分子数据补充了我们的发现，并共同为其分类学提供了新的见解。M.muris的分析基于光学显微镜和扫描电子显微镜。形态学描述集中在两个三叶假牙的牙列模式上。此外,我们描述了外阴的位置，尾部对乳头的排列，来自两性和卵的针状体和测量标本。对于分子系统发育方法，我们扩增了小亚基核糖体RNA基因和内部转录的间隔区，和细胞色素c氧化酶亚基1。基于牙列模式和系统发育聚类的乳鼠形态类型表明该属的细分与其宿主一致。我们认识到两组没有改变正式的分类法：一组包括那些感染MusMusculus的标本，第二组包括感染非Mus啮齿动物的生物。我们的遗传和形态数据揭示了Mastoporus属内的隐秘多样性。我们确定了两个宿主相关的M.muris组。所描述的M.muris的形态类型和基因型允许宿主相关寄生虫之间的一致区分。
