PDF(Pubmed)
Abstract：
Optical emitters in hexagonal boron nitride (hBN) are promising probes for single-molecule sensing platforms. When engineered in nanoparticle form, they can be integrated as detectors in nanodevices, yet positional control at the nanoscale is lacking. Here we demonstrate the functionalization of DNA origami nanopores with optically active hBN nanoparticles (NPs) with nanometer precision. The NPs are active under three wavelengths of visible illumination and display both stable and blinking emission, enabling their accurate localization by using wide-field optical nanoscopy. Correlative opto-structural characterization reveals deterministic binding of bright, multicolor hBN NPs at the pore rim due to π-π stacking interactions at site-specific locations on the DNA origami. Our work provides a scalable, bottom-up approach toward deterministic assembly of solid-state emitters on arbitrary structural elements based on DNA origami. Such a nanoscale arrangement of optically active components can advance the development of single-molecule platforms, including optical nanopores and nanochannel sensors.
摘要：
六方氮化硼（hBN）中的光发射器是用于单分子传感平台的有前途的探针。当设计成纳米粒子时，它们可以作为探测器集成在纳米设备中，然而，在纳米级的位置控制是缺乏。在这里，我们展示了具有纳米精度的光学活性hBN纳米颗粒（NP）的DNA折纸纳米孔的功能化。NP在三个可见光波长下是活跃的，并显示稳定和闪烁的发射，通过使用广域光学纳米显微镜使其精确定位。相关的光学结构表征揭示了明亮的确定性结合，由于DNA折纸上特定位点的π-π堆叠相互作用，孔缘处多色hBNNP。我们的工作提供了一个可扩展的，基于DNA折纸的固态发射器在任意结构元素上的确定性组装的自下而上的方法。光学活性成分的这种纳米级排列可以促进单分子平台的发展，包括光学纳米孔和纳米通道传感器。
